Relationship Between Paraoxonase-1 and Butyrylcholinesterase Activities and Nutritional Status in Mexican Children
| Autor: | Rocío Ramírez-Jiménez, Damianys Almenares-López, Leticia Yáñez-Estrada, María Fernanda Martínez-Salazar, Antonio Monroy-Noyola |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Pediatric Obesity medicine.medical_specialty Antioxidant Genotype Endocrinology Diabetes and Metabolism medicine.medical_treatment Nutritional Status 030204 cardiovascular system & hematology Overweight 03 medical and health sciences 0302 clinical medicine Gene Frequency Internal medicine Internal Medicine medicine Humans Genetic Predisposition to Disease Child Mexico Triglycerides Butyrylcholinesterase chemistry.chemical_classification biology Aryldialkylphosphatase business.industry Paraoxonase Nutritional status medicine.disease PON1 Obesity 030104 developmental biology Endocrinology Enzyme Amino Acid Substitution chemistry Case-Control Studies biology.protein Female medicine.symptom business Polymorphism Restriction Fragment Length |
| Zdroj: | Metabolic Syndrome and Related Disorders. 16:90-96 |
| ISSN: | 1557-8518 1540-4196 |
| Popis: | The enzymes butyrylcholinesterase (BuChE) and paraoxonase-1 (PON1) are the primary bioscavenging enzymes in serum and exhibit antioxidant and anti-inflammatory activities. PON1 has been associated with diseases caused by high oxidative stress, whereas BuChE appears to be involved in the pathophysiology of the metabolic syndrome and related disorders. It has been suggested that children from rural communities in Mexico may have a predisposition to develop obesity or type 2 diabetes during adolescence or adulthood. The objective of this study was to determine whether associations exist between the paraoxonase (PONase)/arylesterase (AREase) activity of PON1, its PON1-Q192R and PON1-L55M polymorphisms, and BuChE activity with the nutritional status and lipid profiles in a group of children from rural communities in Mexico.A group of 97 boys and girls from a rural community in Mexico were assessed for body mass index, the enzymatic activities of BuChE, PONase, and AREase were measured in serum, and their lipid profiles were determined. Genetic polymorphisms of PON1-L55M and PON1-Q192R were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The children were classified into four groups: thinness, normal weight, overweight, and obese. Of the children studied, 34.4% were overweight and obese. The mean age of the participants was 9.5 years (standard deviation = 1.8). The L allele of the PON1-L55M genotype was the most frequent (83.3%), and the R allele of the PON1-Q192R genotype was the most frequent (61.8%). Overweight and obese children had higher values of BuChE, total cholesterol, triglycerides (TG), and lower high-density lipoprotein (HDL-C) values than children with thinness or normal weight (P = 0.028, P = 0.019, P = 0.004, P = 0.069 and P = 0.021, respectively). The levels of AREase and PONase and the prevalence of PON1-L55M and PON1-Q192R genotypes were similar between groups (P = 0.484 and P = 0.380, respectively).This study establishes a positive association of BuChE activity with nutritional status and serum TG. |
| Databáze: | OpenAIRE |
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