Genome-wide identification, classification, and expression of phytocyanins in Populus trichocarpa
| Autor: | Wenfang Hu, Hanwei Yan, Bin Liu, Yue Wang, Luo Shuangshuang, Yan Xiang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Populus trichocarpa Plant Science Biology Genes Plant Real-Time Polymerase Chain Reaction 01 natural sciences Genome Chromosomes Plant 03 medical and health sciences Phylogenetics Metalloproteins Genetics Gene Phylogeny Plant Proteins Synteny Segmental duplication Intron Electron carrier activity biology.organism_classification Populus 030104 developmental biology Transcriptome Copper Genome Plant 010606 plant biology & botany |
| Zdroj: | Planta. 247:1133-1148 |
| ISSN: | 1432-2048 0032-0935 |
| Popis: | 74 phytocyanin genes were identified in the Populus trichocarpa genome. Phylogenetic analysis grouped the PC proteins into four subfamilies (UCs, PLCs, SCs, and ENODLs). Closely related PC proteins share similar motifs, implying similar functions. Expression profiles of PtPC genes were analyzed in response to drought and salt-stress. Phytocyanins (PCs) are blue copper proteins associated with electron carrier activity that have a large influence on plant growth and resistance. The majority of PCs are chimeric arabinogalactan proteins (AGPs). In this work, we identified 74 PC genes in Populus trichocarpa and analyzed them comprehensively. Based on the ligands composition of copper-binding sites, glycosylation state, the domain structure and spectral characteristics of PC genes, PCs were divided into four subfamilies [uclacyanins (UCs), plantacyanins (PLCs), stellacyanins (SCs) and early nodulin-like proteins (ENODLs)], and phylogenetic relationship analysis classified them into seven groups. All PtPCs are randomly distributed on 17 of the 19 poplar chromosomes, and they appear to have undergone expansion via segmental duplication. Eight PtPCs do not contain introns, and each group has a similar conserved motif structure. Promoter analysis revealed cis-elements related to growth, development and stress responses, and established orthology relationships of PCs between Arabidopsis and poplar by synteny analysis. Expression profile analysis and qRT-PCR analysis showed that PtPCs were expressed widely in various tissues. Quantitative real-time RT-PCR analysis of PC genes expression in response to salt and drought stress revealed their stress-responses profiles. This work provides a theoretical basis for a further study of stress resistance mechanisms and the function of PC genes in poplar growth and development. |
| Databáze: | OpenAIRE |
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