Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish, Danio rerio, using transgenics to visualize raphe-specific pet1 expression

Autor: Christian Stigloher, Christina Lillesaar, Birgit Tannhäuser, Laure Bally-Cuif, Mario F. Wullimann
Přispěvatelé: Department of Zebrafish Neurogenetics, Institute of Developmental Genetics, German Research Center for Environmental Health-Helmholtz-Zentrum München (HZM), Graduate School of Systemic Neurosciences, Department Biology II, Neurobiology, Ludwig-Maximilians-Universität München (LMU), Institut de Neurobiologie Alfred Fessard (INAF), Centre National de la Recherche Scientifique (CNRS), Neurobiologie & Développement (N&D)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Male
MESH: Neurons
Animals
Genetically Modified
Mice
0302 clinical medicine
Genes
Reporter
Neural Pathways
MESH: Animals
Transgenes
Zebrafish
Neurons
0303 health sciences
education.field_of_study
biology
General Neuroscience
Brain
MESH: Raphe Nuclei
MESH: Transcription Factors
Female
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Serotonin
MESH: Axons
Population
Green Fluorescent Proteins
Danio
Hindbrain
MESH: Zebrafish Proteins
MESH: Transgenes
Serotonergic
MESH: Animals
Genetically Modified
03 medical and health sciences
MESH: Brain
MESH: Green Fluorescent Proteins
Proto-Oncogene Proteins
Animals
Humans
education
MESH: Zebrafish
MESH: Mice
030304 developmental biology
MESH: Humans
Raphe
MESH: Neural Pathways
MESH: Genes
Reporter
Zebrafish Proteins
biology.organism_classification
Retrograde tracing
Axons
MESH: Male
MESH: Proto-Oncogene Proteins
Raphe Nuclei
MESH: Serotonin
Raphe nuclei
Neuroscience
MESH: Female
030217 neurology & neurosurgery
Transcription Factors
Zdroj: Journal of Comparative Neurology
Journal of Comparative Neurology, Wiley, 2009, 512 (2), pp.158-82. ⟨10.1002/cne.21887⟩
ISSN: 0021-9967
1096-9861
DOI: 10.1002/cne.21887⟩
Popis: International audience; Serotonin is a major central nervous modulator of physiology and behavior and plays fundamental roles during development and plasticity of the vertebrate central nervous system (CNS). Understanding the developmental control and functions of serotonergic neurons is therefore an important task. In all vertebrates, prominent serotonergic neurons are found in the superior and inferior raphe nuclei in the hindbrain innervating most CNS regions. In addition, all vertebrates except for mammals harbor other serotonergic centers, including several populations in the diencephalon. This, in combination with the intricate and wide distribution of serotonergic fibers, makes it difficult to sort out serotonergic innervation originating from the raphe from that of other serotonergic cell populations. To resolve this issue, we isolated the regulatory elements of the zebrafish raphe-specific gene pet1 and used them to drive expression of an eGFP transgene in the raphe population of serotonergic neurons. With this approach together with retrograde tracing we 1) describe in detail the development, anatomical organization, and projection pattern of zebrafish pet1-positive neurons compared with their mammalian counterparts, 2) identify a new serotonergic population in the ventrolateral zebrafish hindbrain, and 3) reveal some extent of functional subdivisions within the zebrafish superior raphe complex. Together, our results reveal for the first time the specific innervation pattern of the zebrafish raphe and, thus, provide a new model and various tools to investigate further the role of raphe serotonergic neurons in vertebrates.
Databáze: OpenAIRE
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