Investigations on the clonality of isolates ofPasteurella gallinarumobtained from turkeys in Germany

Autor:	Klaus-Peter Behr, Henrik Christensen, Gerhard Baron, Jens Peter Christensen, Magne Bisgaard
Rok vydání:	2005
Předmět:	Turkeys HpaII animal diseases Pasteurella Infections Biology Ribotyping Disease Outbreaks Microbiology Food Animals Germany RNA Ribosomal 16S Genotype medicine Animals Typing Respiratory Tract Infections Poultry Diseases General Immunology and Microbiology Outbreak 16S ribosomal RNA medicine.disease Microbial genetics Pasteurella Animal Science and Zoology Pasteurellosis
Zdroj:	Avian Pathology. 34:106-110
ISSN:	1465-3338 0307-9457
DOI:	10.1080/03079450500059412
Popis:	Pasteurella gallinarum has been considered an opportunistic pathogen rather than a primary pathogen for chickens. As P. gallinarum has been found to have a high genotypic diversity, one would expect a polyclonal distribution among isolates from different farms if this organism is a secondary invader. The aims of this investigation were to genetically characterize isolates obtained from outbreaks affecting several turkey farms to confirm the existence of the infection in turkeys and to investigate the genetic relationship between isolates from affected farms. A total of 17 isolates from 14 outbreaks of respiratory disease in Germany were subjected to extended phenotypic and genotypic characterization. All isolates were of the same phenotype, typical of P. gallinarum. Ribotyping of three isolates using either HpaII or HindIII showed that they had identical profiles and indicated that the isolates all originated from the same clone. Comparison with HpaII ribotypes from a previous study showed that the pattern was identical to that obtained with isolates from a Zimbabwean outbreak in chickens during 1999 to 2000. Restriction endonuclease analysis typing of 14 isolates from all 14 farms showed that they had identical profiles but these differed from those obtained with isolates from the Zimbabwean outbreak. Sequencing of the 16S rRNA gene and sequence comparisons with other Pasteurellaceae confirmed their classification as P. gallinarum. Identification of the same clone of P. gallinarum from 14 outbreaks of acute respiratory disease in turkeys within a time period of 2 months suggests a common source of infection, and that P. gallinarum probably played a primary role rather than a secondary role in the outbreaks.
Databáze:	OpenAIRE
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