Evaluation of Antibody Response Directed against Porcine Reproductive and Respiratory Syndrome Virus Structural Proteins
| Autor: | Hiep L.X. Vu, Hung Q Luong, H. T. L. Lai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
animal diseases 030106 microbiology Immunology Virulence lcsh:Medicine Article 03 medical and health sciences antibody profile Antigen luciferase-immunoprecipitation system humoral immunity Drug Discovery medicine Pharmacology (medical) Pharmacology biology medicine.diagnostic_test lcsh:R swine viruses Porcine reproductive and respiratory syndrome virus biology.organism_classification Serum samples Virology 030104 developmental biology Infectious Diseases Antibody response Immunoassay Humoral immunity PRRSV biology.protein Antibody |
| Zdroj: | Vaccines Volume 8 Issue 3 Vaccines, Vol 8, Iss 533, p 533 (2020) |
| ISSN: | 2076-393X |
| DOI: | 10.3390/vaccines8030533 |
| Popis: | Luciferase-immunoprecipitation system (LIPS), a liquid phase immunoassay, was used to evaluate antibody responses directed against the structural proteins of PRRSV in pigs that were experimentally infected with virulent PRRSV strains. First, the viral N protein was used as a model antigen to validate the assay. The LIPS results were highly comparable to that of the commercial IDEXX PRRS X3 ELISA. Subsequently, the assay was applied to simultaneously measure antibody reactivity against all eight structural proteins of PRRSV. The highest immunoreactivities were detected against GP3, M, and N proteins while the lowest reactivity was detected against ORF5a protein. Comparative analysis of the kinetics of antibody appearance revealed that antibodies specific to N protein appeared earlier than antibodies against GP3. Finally, the assay was applied to measure immunoreactivities of clinical serum samples against N and GP3. The diagnostic sensitivity of the LIPS with N protein was superior to that of the LIPS with GP3. Collectively, the results provide additional information about the host antibody response to PRRSV infection. |
| Databáze: | OpenAIRE |
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