MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development
Autor: | Deepika Sharma, Prabodh Kumar Trivedi, Ashutosh Pandey, Aashish Sharma, Chitra Bhatia, Manish Tiwari |
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Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine animal structures Physiology Arabidopsis Down-Regulation Flowers Plant Science 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Botany Gene expression Genetics Metabolomics Arabidopsis thaliana heterocyclic compounds MYB Transcription factor Flavonoids Regulation of gene expression biology Arabidopsis Proteins fungi food and beverages Articles Plants Genetically Modified biology.organism_classification Biosynthetic Pathways Cell biology carbohydrates (lipids) Plant Leaves MicroRNAs Phenotype 030104 developmental biology Flavonoid biosynthesis Seedlings Seeds Flux (metabolism) Transcription Factors 010606 plant biology & botany |
Zdroj: | Plant Physiology. 171:944-959 |
ISSN: | 1532-2548 |
Popis: | MicroRNAs (miRNAs) are endogenous, noncoding small RNAs that function as critical regulators of gene expression. In plants, miRNAs have shown their potential as regulators of growth, development, signal transduction, and stress tolerance. Although the miRNA-mediated regulation of several processes is known, the involvement of miRNAs in regulating secondary plant product biosynthesis is poorly understood. In this study, we functionally characterized Arabidopsis (Arabidopsis thaliana) miR858a, which putatively targets R2R3-MYB transcription factors involved in flavonoid biosynthesis. Overexpression of miR858a in Arabidopsis led to the down-regulation of several MYB transcription factors regulating flavonoid biosynthesis. In contrast to the robust growth and early flowering of miR858OX plants, reduction of plant growth and delayed flowering were observed in Arabidopsis transgenic lines expressing an artificial miRNA target mimic (MIM858). Genome-wide expression analysis using transgenic lines suggested that miR858a targets a number of regulatory factors that modulate the expression of downstream genes involved in plant development and hormonal and stress responses. Furthermore, higher expression of MYBs in MIM858 lines leads to redirection of the metabolic flux towards the synthesis of flavonoids at the cost of lignin synthesis. Altogether, our study has established the potential role of light-regulated miR858a in flavonoid biosynthesis and plant growth and development. |
Databáze: | OpenAIRE |
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