Nucleotide sequence and taxonomic value of the major outer membrane protein gene of Chlamydia pneumoniae IOL-207
| Autor: | S. A. H. Al-Mahdawi, Ian G. Giles, Michael E. Ward, J. D. Treharne, Ian N. Clarke, M. W. Carter |
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| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Restriction Mapping Cloning vector Sequence alignment Biology urologic and male genital diseases Microbiology Plasmid Sequence Homology Nucleic Acid Amino Acid Sequence Chlamydia Cloning Molecular Gene Peptide sequence Phylogeny Genetics Base Sequence Hybridization probe Nucleic acid sequence Exons Biological Evolution Molecular biology female genital diseases and pregnancy complications Blotting Southern genomic DNA Genes Bacterial Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of General Microbiology. 137:465-475 |
| ISSN: | 0022-1287 |
| DOI: | 10.1099/00221287-137-3-465 |
| Popis: | Chlamydia pneumoniae IOL-207 genomic DNA was hybridized with a 1.5 kb labelled DNA probe containing the 3' region of the coding sequence for the major outer membrane protein (MOMP) of C. trachomatis serovar L1. An 8.5 kb Bg/II fragment containing the complete MOMP gene was cloned into lambda EMBL3. Two hybridizing EcoRI fragments were sub-cloned into the lambda ZAP II cloning vector and the resulting plasmids were used as templates for sequencing both strands of the C. pneumoniae MOMP gene. Computer taxonomic studies using the nucleotide and inferred amino acid sequence of the MOMP of C. pneumoniae IOL-207 and all known chlamydial MOMP sequences supported the designation of C. pneumoniae as a new species, but electron microscope studies suggested that the presence of pear-shaped elementary bodies (EBs) may not be a reliable taxonomic criterion. |
| Databáze: | OpenAIRE |
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