Vinclozolin alters the expression of hormonal and stress genes in the midge Chironomus riparius
| Autor: | Paloma Sánchez-Argüello, José-Luis Martínez-Guitarte, Mónica Aquilino |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Midge medicine.medical_specialty Fungicide Endocrine disruption Health Toxicology and Mutagenesis ved/biology.organism_classification_rank.species Endocrine System Genes Insect 010501 environmental sciences Aquatic Science Biology Stress 01 natural sciences Chironomidae 03 medical and health sciences chemistry.chemical_compound Internal medicine Gene expression medicine Animals HSP70 Heat-Shock Proteins Vinclozolin Oxazoles Gene 0105 earth and related environmental sciences Chironomus riparius Regulation of gene expression ved/biology Enzymes Cell biology Enzyme Activation Metabolic pathway 030104 developmental biology Endocrinology Gene Expression Regulation chemistry Larva Water Pollutants Chemical Ecdysone Hormone |
| Zdroj: | Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA INIA: Repositorio de Resultados de Investigación del INIA |
| ISSN: | 0166-445X |
| Popis: | Vinclozolin is a fungicide used in agriculture that can reach aquatic ecosystems and affect the organisms living there. Its effects have been intensively studied in vertebrates, where it acts as an antiandrogen, but there is a lack of information about its mechanistic effects on invertebrates. In this work, we analyzed the response of genes related to the endocrine system, the stress response, and the detoxification mechanisms of Chironomus riparius fourth instar larvae after 24 h and 48 h exposures to 20 (69.9 nM), 200 (699 nM), and 2000 μg/L (6.99 μM) of Vinclozolin. Survival analysis showed that this compound has low toxicity, as it was not lethal for this organism at the concentrations used. However, this fungicide was shown to modify the transcriptional activity of the ecdysone response pathway genes EcR, E74, and Kr-h1 by increasing their mRNA levels. While no changes were observed in disembodied, a gene related with the ecdysone synthesis metabolic pathway, Cyp18A1, which is involved in the inactivation of the active form of ecdysone, was upregulated. Additionally, the expression of two genes related to other hormones, FOXO and MAPR, did not show any changes when Vinclozolin was present. The analysis of stress response genes showed significant changes in the mRNA levels of Hsp70, Hsp24, and Gp93, indicating that Vinclozolin activates the cellular stress mechanisms. Finally, the expressions of the genes Cyp4G and GstD3, which encode enzymes involved in phase I and phase II detoxification, respectively, were analyzed. It was found that their mRNA levels were altered by Vinclozolin, suggesting their involvement in the degradation of this compound. For the first time, these results show evidence that Vinclozolin can modulate gene expression, leading to possible significant endocrine alterations of the insect endocrine system. These results also offer new clues about the mode of action of this compound in invertebrates. © 2016 Elsevier B.V.. |
| Databáze: | OpenAIRE |
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