Localization of a Calcium Sensitive Binding Site for Gelsolin on Actin Subdomain I : Implication for Severing Process

Autor:	Claude Roustan, Marie-christine Lebart, Jeanne Feinberg, Yves Benyamin
Rok vydání:	1997
Předmět:	Binding Sites biology Chemistry Phalloidin Biophysics Arp2/3 complex Actin remodeling macromolecular substances Cell Biology Biochemistry Actins chemistry.chemical_compound biology.protein Animals Calcium Rabbits MDia1 Actin-binding protein Binding site Molecular Biology Gelsolin Actin
Zdroj:	Biochemical and Biophysical Research Communications. 233:61-65
ISSN:	0006-291X
Popis:	The binding of the N-terminal domain (S1) of gelsolin to monomeric actin has been extensively documented. In contrast, the location of the C-terminal calcium dependent domains (S4-6) interacting with the actin filament during the severing process remains uncertain. In this study, we have identified a new interface that supports calcium dependent gelsolin binding to actin. This site is located in a critical position towards actin-actin contact in the filament and in the vicinity of the phalloidin site. Using specific antibody and synthetic peptides derived from actin sequence within 105-132 residues, this interface was finally ascribed to the segment 112-120 on the actin subdomain-1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e179b35aa741eca2764b89ebbd315b73 https://doi.org/10.1006/bbrc.1997.6402 Zobrazit plný text záznamu Full Text from ScienceDirect