Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
| Autor: | Urara Ota, Inoru Yokoe, Norihiko Ito, Liming Li, Masahiro Ishizuka, Kiwamu Takahashi, Hideo Imazato, Yoshiharu Okamoto, Takeshi Tsuka, Tomohiro Osaki, Tomoki Ichikawa, Kazuo Azuma, Yusuke Murahata, Tomohiro Imagawa, Masamichi Yamashita, Takuya Ishii, Tohru Tanaka, Yuji Sunden |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
030103 biophysics Cancer Research Veterinary medicine sarcoma medicine.medical_treatment cat Photodynamic therapy carcinoma lcsh:RC254-282 Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine polycyclic compounds medicine protoporphyrin IX Photosensitizer Chemotherapy Protoporphyrin IX biology Chemistry Ferrochelatase medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Primary tumor eye diseases Oncology 5-aminolevulinic acid 030220 oncology & carcinogenesis dog biology.protein Sarcoma Intracellular |
| Zdroj: | Cancers Cancers, Vol 11, Iss 4, p 495 (2019) Volume 11 Issue 4 |
| ISSN: | 2072-6694 |
| Popis: | 5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/&minus chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine. |
| Databáze: | OpenAIRE |
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