Growth Hormones Reverse Desensitization of P2Y2 Receptors in Rat Mesangial Cells
| Autor: | Avi Ring, Xiaofen Lou, G. Persson, A. Erik, A.M. Gutiérrez |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor Glomerular Mesangial Cell medicine.medical_treatment Becaplermin Biophysics Uridine Triphosphate Biology Biochemistry Rats Sprague-Dawley Receptors Purinergic P2Y2 chemistry.chemical_compound Adenosine Triphosphate Alkaloids Internal medicine medicine Animals Insulin Enzyme Inhibitors Receptor Molecular Biology Cells Cultured Protein Kinase C Protein kinase C Desensitization (medicine) Benzophenanthridines Platelet-Derived Growth Factor Receptors Purinergic P2 Purinergic receptor Proto-Oncogene Proteins c-sis Cell Biology Glomerular Mesangium Phenanthridines Rats Endocrinology Chelerythrine chemistry Calcium Signal transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 270:594-599 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2000.2461 |
| Popis: | Rat glomerular mesangial cells (GMC) express P2Y(2) purinoceptors and respond to nucleotide stimuli with a transient increase in the cytosolic Ca(2+) concentration and the receptors desensitize upon repeated stimulation with nucleotide. We demonstrate that there is a cross-talk from the signaling of tyrosine kinase to P2Y(2) receptors. For most cells repeated applications of ATP completely abolished the response, as did activation of PKC with 500 nM PMA. In contrast, preincubation with the PKC inhibitor chelerythrine (100 nM) prevented desensitization. Desensitization after application of ATP was reversed by subsequent incubation with PDGF-BB (50 ng/ml) or insulin (660 mU/ml). We conclude that the desensitization is caused by phosphorylation due to PKC and is under the control of growth factors. The findings support the hypothesis that growth hormones potentiate nucleotides as proinflammatory mediators and we hypothesize that they have bearing on the hyperfiltration seen in diabetes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect