Experimental Model of Naturally Occurring Post-radiation Sarcoma: Interest of Positron Emission Tomography (PET) for Early Detection

Autor: Pierre-Yves Marie, Gilles Dolivet, Patrice Gallet, Pierre Bravetti, Bérengère Phulpin, Sylvain Poussier, Claire Blaise, Philippe Henrot, S. Huger, Jean-Louis Merlin, Sophie Pinel, Nguyen Tran, Agnès Leroux, Pierre Graff
Přispěvatelé: Centre Alexis Vautrin (CAV), Signalisation, Génomique et Recherche Translationnelle en Oncologie (SIGRETO), Université Henri Poincaré - Nancy 1 (UHP), École de chirurgie, faculté de médecine de Nancy, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Risque cardiovasculaire, rigidité-fibrose et hypercoagulabilité (RCV), Université Henri Poincaré - Nancy 1 (UHP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Automatique de Nancy (CRAN), Université Henri Poincaré - Nancy 1 (UHP)-Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2012
Předmět:
Male
MESH: Neoplasms
Radiation-Induced
Fluorine Radioisotopes
Neoplasms
Radiation-Induced
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Soft Tissue Neoplasms
Hindlimb
MESH: Sarcoma
Experimental
030218 nuclear medicine & medical imaging
Mice
0302 clinical medicine
MESH: Early Diagnosis
MESH: Fluorodeoxyglucose F18
Medicine
MESH: Animals
Cobalt Radioisotopes
MESH: Cobalt Radioisotopes
Radiation
medicine.diagnostic_test
MESH: Hindlimb
MESH: Positron-Emission Tomography
3. Good health
MESH: Models
Animal
Positron emission tomography
030220 oncology & carcinogenesis
Models
Animal
Sarcoma
Experimental
Sarcoma
MESH: Tomography
X-Ray Computed
MESH: Radiopharmaceuticals
MESH: Soft Tissue Neoplasms
MESH: Rats
Mice
Nude
Early detection
[SDV.CAN]Life Sciences [q-bio]/Cancer
03 medical and health sciences
Fluorodeoxyglucose F18
MESH: Mice
Nude
Biomarkers
Tumor
Animals
Potency
Neoplasm Invasiveness
Radiology
Nuclear Medicine and imaging
Rats
Wistar
Adverse effect
MESH: Mice
MESH: Fluorine Radioisotopes
Serial Transplantation
business.industry
MESH: Neoplasm Invasiveness
MESH: Rats
Wistar
medicine.disease
MESH: Male
Rats
Radiation therapy
Early Diagnosis
Positron-Emission Tomography
MESH: Tumor Markers
Biological
Radiopharmaceuticals
Tomography
X-Ray Computed
business
Nuclear medicine
MESH: Neoplasm Transplantation
Neoplasm Transplantation
Zdroj: Journal of Radiation Research
Journal of Radiation Research, Oxford University Press (OUP), 2012, 53 (1), pp.101-109. ⟨10.1269/jrr.11008⟩
ISSN: 1349-9157
0449-3060
DOI: 10.1269/jrr.11008
Popis: International audience; Radiotherapy is an integral part of overall cancer therapy. One of the most serious adverse effects of irradiation concern, for long-term survivors, the development of post-radiation sarcoma (PRS) in healthy tissues located within the irradiated area. PRS have bad prognosis and are often detected at a late stage. Therefore, it is obvious that the early detection PRS is a key-point and the development of preclinical models is worthy to evaluate innovative diagnostic and therapeutic procedures. The aim of this study was to develop a spontaneous rodent model of PRS and to evaluate the potency of Positron Emission Tomography (PET) for early detection. Fifteen Wistars rats were irradiated unilateraly on the hindlimb with a single dose of 30 Gy. Sequential analysis was based on observational staging recordings, Computerized Tomography (CT) scanning and PET. Tumors were removed and, histopathological and immunochemistry analyses were performed. Among the irradiated rats, 12 sarcomas (80%) were detected. All tumors occurred naturallty within the irradiated hindlimb and were highly aggressive since most tumors (75%) were successfully transplanted and maintained by serial transplantation into nude mice. Upon serial staging recordings, using PET, was found to enable the detection of PRS earlier after irradiation than with the other methods (i.e. 11.9 ± 1.8 vs 12.9 ± 2.6 months). These results confirmed the interest of experimental models of PRS for the preclinical evaluation of innovative diagnostic strategies and confirmed the potency of PET for early detection of PRS. This preclinical model of PRS can also be proposed for the evaluation of therapeutic strategies.
Databáze: OpenAIRE
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