Genetic Characterization and Comparative Genome Analysis ofBrucella melitensisIsolates from India
| Autor: | Sashi Bhushan Rao, Sarwar Azam, Veera NarasimhaRao, Padmaja Jakka, Bindu Bhargavi, Girish K. Radhakrishnan, Vivek Kumar Gupta |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Whole genome sequencing Article Subject lcsh:QH426-470 biology Pharmaceutical Science Brucellosis Brucella biology.organism_classification medicine.disease Biochemistry Virology Genome lcsh:Genetics 03 medical and health sciences 030104 developmental biology medicine Multilocus sequence typing Typing Molecular Biology Prophage Research Article Brucella melitensis |
| Zdroj: | International Journal of Genomics International Journal of Genomics, Vol 2016 (2016) |
| ISSN: | 2314-4378 2314-436X |
| DOI: | 10.1155/2016/3034756 |
| Popis: | Brucellosis is the most frequent zoonotic disease worldwide, with over 500,000 new human infections every year.Brucella melitensis, the most virulent species in humans, primarily affects goats and the zoonotic transmission occurs by ingestion of unpasteurized milk products or through direct contact with fetal tissues. Brucellosis is endemic in India but no information is available on population structure and genetic diversity ofBrucellaspp. in India. We performed multilocus sequence typing of fourB.melitensisstrains isolated from naturally infected goats from India. For more detailed genetic characterization, we carried out whole genome sequencing and comparative genome analysis of one of theB.melitensisisolates, Bm IND1. Genome analysis identified 141 unique SNPs, 78 VNTRs, 51 Indels, and 2 putative prophage integrations in the Bm IND1 genome. Our data may help to develop improved epidemiological typing tools and efficient preventive strategies to control brucellosis. |
| Databáze: | OpenAIRE |
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