Enzymatic grafting of simple phenols on flax and sisal pulp fibres using laccases
| Autor: | Ángel T. Martínez, Ana Gutiérrez, Amanda Fillat, José F. Colom, Teresa Vidal, Elisabetta Aracri, José C. del Río |
|---|---|
| Přispěvatelé: | Ministerio de Educación y Ciencia (España), European Commission |
| Rok vydání: | 2010 |
| Předmět: |
Environmental Engineering
Sinapaldehyde Bioengineering engineering.material Syringaldehyde Gas Chromatography-Mass Spectrometry Ferulic acid chemistry.chemical_compound Agave Phenols stomatognathic system Flax Organic chemistry Non-wood fibres Coloring Agents Functionalization Waste Management and Disposal SISAL computer.programming_language Trametes Laccase Grafting biology Renewable Energy Sustainability and the Environment Pulp (paper) General Medicine Pycnoporus cinnabarinus biology.organism_classification Pycnoporus Biodegradation Environmental chemistry engineering Natural phenols computer |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0960-8524 2007-6800 |
| DOI: | 10.1016/j.biortech.2010.05.080 |
| Popis: | Textile and Paper Engineering Department. ETSEIAT. Universitat Politècnica de Catalunya. Colom 11. E-08222 Terrassa, Spain Instituto de Recursos Naturales y Agrobiología de Sevilla, CSIC, P.O. Box 1052. E-41080 Seville, Spain Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9. E-28040 Madrid, Spain Flax and sisal pulps were treated with two laccases (from Pycnoporus cinnabarinus, PcL and Trametes villosa, TvL respectively), in the presence of different phenolic compounds (syringaldehyde, acetosyringone and p-coumaric acid in the case of flax pulp, and coniferaldehyde, sinapaldehyde, ferulic acid and sinapic acid in the case of sisal pulp). In most cases the enzymatic treatments resulted in increased kappa number of pulps suggesting the incorporation of the phenols into fibres. The covalent binding of these compounds to fibres was evidenced by the analysis of the treated pulps, after acetone extraction, by pyrolysis coupled with gas chromatography/mass spectrometry in the absence and/or in the presence of tetramethylammonium hydroxide (TMAH) as methylating agent. The highest extents of phenol incorporation were observed with the p-hydroxycinnamic acids p-coumaric and ferulic acids. The present work shows for the first time the use of analytical pyrolysis as an effective approach to study fibre functionalisation by laccase-induced grafting of phenols. This work was supported by a Spanish MEC projects TECHFIBER (CTQ2007-68003-C02-01), FUNCICEL (CTQ2009-12904) and ELLE (AGL2008-00709) and a European Integrated Project from Sixth Framework Program BIORENEW (NMP2-CT-2006-026456). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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