A laboratory bioassay method to assess the use of Toxic bait on anastrepha fraterculus (Weidemann 1830)
| Autor: | Cléber Antonio Baronio, Daniel Bernardi, Marcos Botton, Morgana Mattiello Baldin, J Pasinato, Marcelo Zanelato Nunes |
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| Přispěvatelé: | Marcelo Z. Nunes, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil, Daniel Bernardi, Dept of Entomology, Embrapa Clima Temperado, Pelotas, RS, Brazil, Cléber A. Baronio, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil, Joel Pasinato, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brazil, Morgana M. Baldin, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brazil, MARCOS BOTTON, CNPUV. |
| Jazyk: | portugalština |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Food deprivation Insecticides Time Factors Spinosad Biology South American fruit fly 010603 evolutionary biology 01 natural sciences Insect Control Toxicology Frui fly Toxicity Tests medicine Bioassay Animals Mango fruit Larva Tephritidae Methodology Anastrepha fraterculus 010602 entomology Drug Combinations Insect Science South american Toxicity Biological Assay Macrolides medicine.drug |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| Popis: | The lack of standardization of bioassays for the evaluation of toxic baittoxicity on the South American fruit fly,Anastrepha fraterculus(Weidemann 1830), has led to erroneous interpretations of assay results.The objective of this study was to develop a methodology for the stan-dardization and validation of toxicological tests onA. fraterculustoxic baitusing the Success?0.02CB formulation (80 mg L−1of spinosad).Anastrepha fraterculusadults, obtained from larvae reared on an artificialdiet, showed higher susceptibility (LT50= 48.96 h) than adults from larvaereared on cattley guava (LT50= 53.83 h) and mango fruit (LT50= 53.55 h).Anastrepha fraterculusadults at the age of five (LT50= 65.30 h), 15 (LT50=59.01 h), and 30 (LT50= 55.53 h) days presented similar toxicity. Theconsumption of toxic bait (4.74 mg) increased at 15 days, a fact alsoobserved with insects without food deprivation. In addition, the absenceof a food source (artificial diet) with the toxic bait significantly reducedadult mortality time by 7 h (LT50= 57.42 h). In relation to exposure time,adults exposed to toxic bait for 1 h reduced consumption by 25%; however,they showed the same susceptibility as insects exposed to 2 (LT50=55.72h), 4 (LT50=57.64h),and8h(LT50= 57.76 h). However, with 24 h of fooddeprivation, they had a higher susceptibility (LT50= 46.48 h). Five-day-oldA. fraterculusadults fed an artificial diet before being deprived of food for12 or 24 h, then exposed to toxic bait for 4 h in the absence of a foodsource, are considered optimum conditions to evaluate the toxicity oftoxic bait. Made available in DSpace on 2019-11-20T18:12:39Z (GMT). No. of bitstreams: 1 Nunesetal2019ALaboratoryBioassayMethodtoAssesstheUseofToxic.pdf: 671018 bytes, checksum: 40a4fc6ea6581cf21f9ea56a58ef7fe2 (MD5) Previous issue date: 2019 |
| Databáze: | OpenAIRE |
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