Antioxidant activity, phytochemical composition of Andricus tomentosus and its antiproliferative effect on Mia-Paca2 cell line
| Autor: | Mücahit Seçme, Ramazan Mammadov, Özge Kilinçarslan Aksoy |
|---|---|
| Přispěvatelé: | MÜ, Fen Fakültesi, Moleküler Biyoloji Ve Genetik Bölümü, Mammadov, Ramazan |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Andricus tomentosus Antioxidant antioxidant DPPH caspase 8 gene medicine.medical_treatment Phytochemicals Wasps Flavonoid aurantiin antioxidant activity IC50 TRADD gene FADD gene Bcl 2 gene Antioxidants tannin Quercus chemistry.chemical_compound 0302 clinical medicine 4 hydroxybenzoic acid molybdenum caspase 9 gene Galls plant medicinal product Bax gene Caffeic acid Gall phenol antineoplastic agent chemistry.chemical_classification ABTS radical scavenging assay Andricus tomentosus extract beta carotene ABTS Traditional medicine Chemistry caspase 10 gene drug cytotoxicity apoptosis General Medicine rutoside unclassified drug caspase 3 gene 030220 oncology & carcinogenesis MIA PaCa-2 cell line gallic acid coumaric acid Ellagic acid linoleic acid antiproliferative activity in vitro study high performance liquid chromatography Linoleic acid chlorogenic acid XTT FAS gene 3 4 dihydroxy benzoic acid cell survival Article 03 medical and health sciences ellagic acid Cell Line Tumor oak Genetics medicine Animals Humans flavonoid gene Molecular Biology 52 5 dihydroxy benzoic acid Cell Proliferation BID gene epicatechin Pancreatic Neoplasms drug efficacy 030104 developmental biology vanillic acid phosphomolybdenum DPPH radical scavenging assay phytochemistry HPLC caffeic acid cinnamic acid ferulic acid |
| Popis: | Kilincarslan Aksoy, Ozge/0000-0003-4944-1029 WOS: 000573399400001 PubMed ID: 32986201 Plant derived products are widely used in cancer treatment. Gall species has been preferred for treatment various diseases in folk medicine, but there are few studies on the anticancer effects of gall species. The present study reports the antioxidant activity and total secondary metabolites of extracts ofA. tomentosusgalls. The antioxidant potency of galls was carried out using differentin-vitromodel systems. Their cytotoxic efficacy on Mia-Paca cell line was also explored. Gall extract was found to contain a large amount of phenolic acids. The extract potently scavenged free radicals including DPPH (IC(50)9.56 +/- 1.08 mu g/mL), ABTS (IC(50)18.51 +/- 0.25 mu g/mL). It can be seen as a potential source of antioxidants according to beta-carotene/linoleic acid method (%92.58 +/- 0.92) and Phosphomolybdenum assays (104.36 +/- 4.95 mgAE/g). Gall extract also posses ability of metal chelating (%40.07 +/- 2.30) and Fe3+(184.01 +/- 2.83 mgTE/g) and Cu2+(89.81 +/- 0.96 mgTE/g) reducing activity. According to total secondary metabolites tests, gall extract showed high total phenolic, total flavonoid and total tannin amount. HPLC analysis of the extract suggested it to contain caffeic acid (424.068.479 mu g/g), ellagic acid (187.696.132 mu g/g). XTT assay revealed gall extract to enhance percent survival of Mia-Paca2 cell line exposedA. tomentosusextracts. The best cytotoxic effect was determined in acetone extracts (IC50: 124.7 mu M). Expression of some genes (Bax, Bcl-2, FAS, BID, caspase-3, caspase-8, caspase-9, caspase-10, FADD, TRADD) in the apoptosis pathway was determined to invastigate apoptosis inducing activity. These results indicate thatA. tomentosusgalls possess potent antioxidant activity, when tested both in chemical as well as biological models. Pamukkale University Scientific Research Projects Coordination UnitPamukkale University [2018FEBE062] The authors are grateful for the financial support from the Pamukkale University Scientific Research Projects Coordination Unit (Project No: 2018FEBE062). |
| Databáze: | OpenAIRE |
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