A boundary delimitation algorithm to approximate cell soma volumes of bipolar cells from topographical data obtained by scanning probe microscopy
| Autor: | Kerstin Möller, Patrick Happel, Irmgard D. Dietzel, Ralf Kunz |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Cell
Image processing Biology Microscopy Scanning Probe lcsh:Computer applications to medicine. Medical informatics Biochemistry Scanning probe microscopy Structural Biology Position (vector) Cell Movement Microscopy medicine Image Processing Computer-Assisted Animals lcsh:QH301-705.5 Molecular Biology Cell Size Cell Nucleus Applied Mathematics Methodology Article Stem Cells Rats Computer Science Applications Oligodendroglia medicine.anatomical_structure lcsh:Biology (General) Scanning ion-conductance microscopy lcsh:R858-859.7 Soma Algorithm Algorithms Volume (compression) |
| Zdroj: | BMC Bioinformatics, Vol 11, Iss 1, p 323 (2010) BMC Bioinformatics |
| ISSN: | 1471-2105 |
| DOI: | 10.1186/1471-2105-11-323 |
| Popis: | Background Cell volume determination plays a pivotal role in the investigation of the biophysical mechanisms underlying various cellular processes. Whereas light microscopy in principle enables one to obtain three dimensional data, the reconstruction of cell volume from z-stacks is a time consuming procedure. Thus, three dimensional topographic representations of cells are easier to obtain by scanning probe microscopical measurements. Results We present a method of separating the cell soma volume of bipolar cells in adherent cell cultures from the contributions of the cell processes from data obtained by scanning ion conductance microscopy. Soma volume changes between successive scans obtained from the same cell can then be computed even if the cell is changing its position within the observed area. We demonstrate that the estimation of the cell volume on the basis of the width and the length of a cell may lead to erroneous determination of cell volume changes. Conclusions We provide a new algorithm to repeatedly determine single cell soma volume and thus to quantify cell volume changes during cell movements occuring over a time range of hours. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|