Studies on the Mechanism of Replication of Adenovirus DNA II. The Nature of Single-Stranded DNA in Replicative Intermediates
| Autor: | John S. Sussenbach, H. S. Jansz, D. J. Ellens |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
DNA Replication
Sucrose Semiconservative replication DNA polymerase DNA polymerase II Immunology Cesium DNA Single-Stranded Eukaryotic DNA replication Tritium Virus Replication Microbiology Adenoviridae Cell Line Adenosine Triphosphate Replication factor C Control of chromosome duplication Virology Animal Viruses Centrifugation Density Gradient Humans Cell Nucleus Chromatography biology Carcinoma DNA replication Nucleic Acid Hybridization Molecular biology Microscopy Electron Insect Science DNA Viral biology.protein DNA supercoil Mouth Neoplasms Phosphorus Radioisotopes |
| Zdroj: | Journal of Virology. 12:1131-1138 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.12.5.1131-1138.1973 |
| Popis: | Replicative intermediates of adenovirus type 5 DNA contain large stretches of single-stranded DNA. We have shown that this single-stranded DNA is mainly of parental origin, whereas all new DNA synthesized during one round of replication has a double-stranded structure. Hybridization experiments of the single-stranded DNA with isolated complementary strands of adenovirus type 5 DNA showed that this DNA hybridized only with the viral L-strand (the strand with the lower equilibrium density in alkaline CsCl) indicating that it represents the viral H-strand. This observation implies that replication always starts from one and the same molecular end. Electron microscopy of partially denatured Y-shaped intermediates confirmed this and showed that replication started from the molecular right end (the end richest in A-T base pairs). In conclusion, we have shown that replication of adenovirus type 5 DNA starts at the molecular right end, displacing the parental H-strand. |
| Databáze: | OpenAIRE |
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