Misleading de novo detection of serum anti-HLA-A3 antibodies in kidney recipients having received ATG before transplantation

Autor: Chantal Thevenin, Pierre Tiberghien, Jean-Michel Rebibou, Maria Yannaraki, Emeline Masson, Jacqueline Chabod, Nadège Devillard
Přispěvatelé: Saas, Philippe, Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire d'Immunogénétique, Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Centre d'Investigation Clinique de Besançon (Inserm CIC 1431), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Service d'urologie, andrologie et transplantation rénale, Hôpital Saint-Jacques-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Hôpital Saint-Jacques
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Male
Transplantation Conditioning
030232 urology & nephrology
MESH: Rabbits
MESH: Flow Cytometry
Cell Separation
HLA-A3 Antigen
030230 surgery
MESH: Kidney Transplantation
0302 clinical medicine
MESH: Antilymphocyte Serum
Immunology and Allergy
MESH: Animals
MESH: Transplantation Conditioning
Kidney
medicine.diagnostic_test
General Medicine
Flow Cytometry
3. Good health
medicine.anatomical_structure
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
Rabbits
Antibody
Adult
[SDV.IMM] Life Sciences [q-bio]/Immunology
T cell
Immunology
Human leukocyte antigen
Biology
MESH: Cell Separation
Antibodies
Flow cytometry
03 medical and health sciences
HLA-A3
medicine
Animals
Humans
Antilymphocyte Serum
MESH: Humans
MESH: Antibodies
MESH: Adult
Kidney Transplantation
MESH: Male
MESH: HLA-A3 Antigen
Transplantation
Polyclonal antibodies
biology.protein
MESH: Female
Zdroj: Human Immunology
Human Immunology, Elsevier, 2010, 71 (2), pp.170-5. ⟨10.1016/j.humimm.2009.11.003⟩
ISSN: 0198-8859
DOI: 10.1016/j.humimm.2009.11.003⟩
Popis: International audience; Anti-HLA antibody (Ab) monitoring is essential for the follow-up of transplant patients. However, it can be affected by drugs, especially Ab infused as a conditioning regimen for transplantation. ATG Fresenius, commonly used in this setting, is a polyclonal rabbit Ab raised against the Jurkat human T cell line (HLA-A3, 32; B7, 35). We report here the de novo detection by CDC and flow cytometry (Luminex) of anti-HLA-A3 Ab in the serum of kidney recipients treated with ATG Fresenius. The Ab, of rabbit origin, was detected in every assessable patient (n = 16), with the exception of the HLA-A3 recipients and/or recipients receiving an HLA-A3 graft, before becoming undetectable, at latest, at day 102 after transplantation. It is of major importance that transplantation monitoring laboratories bear in mind the possibility of therapeutic Ab detection when interpreting anti-HLA Ab results.
Databáze: OpenAIRE
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