Rethinking Breast Cancer Chemoprevention: Technological Advantages and Enhanced Performance of a Nanoethosomal-Based Hydrogel for Topical Administration of Fenretinide
| Autor: | Alexsandra Conceição Apolinário, Giovanna Cassone Salata, Marcelo Medina de Souza, Marlus Chorilli, Luciana Biagini Lopes |
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| Přispěvatelé: | Universidade de São Paulo (USP), Butantan Institute, Universidade Estadual Paulista (UNESP) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Bioadhesion
Ecology Fenretinide Skin Absorption Pharmaceutical Science Breast Neoplasms Hydrogels General Medicine Aquatic Science Administration Cutaneous NEOPLASIAS MAMÁRIAS Drug Delivery Systems Nanomedicine Drug Discovery Liposomes Transdermal hydrogel Animals Humans Female Retinoid Agronomy and Crop Science Chickens Ecology Evolution Behavior and Systematics Skin |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Made available in DSpace on 2022-05-01T16:48:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-05-01 Herein, we developed an ethosomal hydrogel based on three types of ethosomes: simple, mixed (surfactant-based micelles and lipid vesicles) or binary (comprising two type of alcohols). Ethanol injection was employed for vesicles preparation, and sodium alginate, as gelling agent. We purposed the local-transdermal administration of the off-the-shelf retinoid fenretinide (FENR) for chemoprevention of breast cancer. Rheograms and flow index values for alginate dispersion (without ethosomes) and hydrogels containing simple, mixed or binary ethosomes suggested pseudoplastic behavior. An increase in the apparent viscosity was observed upon ethosome incorporation. The ethosomal hydrogel displayed increased bioadhesion compared to the alginate dispersion, suggesting that the lipid vesicles contribute to the gelling and bioadhesion processes. In the Hen’s Egg Test–Chorioallantoic Membrane model, few spots of lysis and hemorrhage were observed for formulations containing simple (score of 2) and mixed vesicles (score 4), but not for the hydrogel based on the binary system, indicating its lower irritation potential. The binary ethosomal hydrogel provided a slower FENR in vitro release and delivered 2.6-fold less drug into viable skin layers compared to the ethosome dispersion, supporting the ability of the gel matrix to slow down drug release. The ethosomal hydrogel decreased by ~ five-fold the IC50 values of FENR in MCF-7 cells. In conclusion, binary ethosomal gels presented technological advantages, provided sustained drug release and skin penetration, and did not preclude drug cytotoxic effects, supporting their potential applicability as topical chemopreventive systems. Department of Pharmacology Institute of Biomedical Sciences University of São Paulo, Av. Prof. Lineu Prestes, 1524, SP Centre of Excellence in New Target Discovery (CENTD) Butantan Institute School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo |
| Databáze: | OpenAIRE |
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