Functional and Molecular Analysis of the Double-Positive Stage-Specific CD8 Enhancer E8III during Thymocyte Development
| Autor: | Ivan Bilic, Dan R. Littman, Bernd Unger, Johanna Tinhofer, Nicholas Feik, Wilfried Ellmeier |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
CD8 Antigens
T cell Immunology Mice Transgenic Enhancer RNAs Thymus Gland Biology Cell Line E-Box Elements Mice Proto-Oncogene Proteins c-myb T-Lymphocyte Subsets medicine Animals Humans Immunology and Allergy Intestinal Mucosa Promoter Regions Genetic Enhancer Regulation of gene expression Binding Sites Gene targeting Cell Differentiation Dendritic Cells Molecular biology CD8A Mice Inbred C57BL Thymocyte Enhancer Elements Genetic medicine.anatomical_structure Gene Expression Regulation CD4 Antigens Gene Targeting Gene Deletion CD8 |
| Zdroj: | The Journal of Immunology. 174:1513-1524 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Several developmental stage-, subset-, and lineage-specific Cd8 cis-regulatory regions have been identified. These include the E8III enhancer, which directs expression in double-positive (DP) thymocytes, and E8II, which is active in DP cells and CD8+ T cells. Using a transgenic reporter expression assay, we identified a 285-bp core fragment of the E8III enhancer that retains activity in DP thymocytes. In vitro characterization of the core enhancer revealed five regulatory elements that are required for full enhancer activity, suggesting that multiple factors contribute to the developmental stage-specific activity. Furthermore, deletion of E8III in the mouse germline showed that this enhancer is required for nonvariegated expression of CD8 in DP thymocytes when E8II is also deleted. These results indicate that E8III is one of the cis-elements that contribute to the activation of the Cd8a and Cd8b gene complex during T cell development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|