The Systematic Effect of Mesenchymal Stem Cell Therapy in Critical COVID-19 Patients: A Prospective Double Controlled Trial

Autor: Zuhal Yesilbag, P Kasapoglu, Nilgun Isiksacan, Z. Cukurova, K Kart Yasar, Erdal Karaoz, Ravza Yilmaz, Gokhan Adas, I D Koyuncu
Přispěvatelé: İstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Koyuncu Irmak, Duygu, Karaoz, Erdal
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Chemokine
Severity of Illness Index
Gastroenterology
Interferon gamma
Lymphocytes
Prospective Studies
biology
Stem Cells
Middle Aged
cytokines
growth factors
Interleukin-10
Interleukin 10
C-Reactive Protein
Treatment Outcome
Mesenchymal Stem Cell
Cytokines
Medicine
Female
Original Article
Chemokines
medicine.drug
Adult
medicine.medical_specialty
Critical Illness
Biomedical Engineering
Cell Based Therapies
Mesenchymal Stem Cell Transplantation
Proinflammatory cytokine
Interferon-gamma
Immune Regulation and Therapy
Internal medicine
Growth Factors
medicine
Humans
Transplantation
SARS-CoV-2
business.industry
Interleukin-8
C-reactive protein
Mesenchymal stem cell
COVID-19
Mesenchymal Stem Cells
Cell Biology
medicine.disease
Viral Infections
biology.protein
Leukocyte Common Antigens
business
Cytokine storm
Cytokines and Mediators
Zdroj: Cell Transplantation, Vol 30 (2021)
Cell Transplantation
ISSN: 1555-3892
Popis: The aim of this clinical trial was to control the cytokine storm by administering mesenchymal stem cells (MSCs) to critically-ill COVID-19 patients, to evaluate the healing effect, and to systematically investigate how the treatment works. Patients with moderate and critical COVID-19 clinical manifestations were separated as Group 1 (moderate cases, n = 10, treated conventionally), Group 2 (critical cases, n = 10, treated conventionally), and Group 3 (critical cases, n = 10, treated conventionally plus MSCs transplantation therapy of three consecutive doses on treatment days 0, 3, and 6, (as 3 × 106 cells/kg, intravenously). The treatment mechanism of action was investigated with evaluation markers of the cytokine storm, via biochemical parameters, levels of proinflammatory and anti-inflammatory cytokines, analyses of tissue regeneration via the levels of growth factors, apoptosis markers, chemokines, matrix metalloproteinases, and granzyme-B, and by the assessment of the immunomodulatory effects via total oxidant/antioxidant status markers and the levels of lymphocyte subsets. In the assessment of the overall mortality rates of all the cases, six patients in Group-2 and three patients in Group-3 died, and there was no loss in Group-1. Proinflammatory cytokines IFNγ, IL-6, IL-17A, IL-2, IL-12, anti-inflammatory cytokines IL-10, IL-13, IL-1ra, and growth factors TGF-β, VEGF, KGF, and NGF levels were found to be significant in Group-3. When Group-2 and Group-3 were compared, serum ferritin, fibrinogen and CRP levels in Group-3 had significantly decreased. CD45 +, CD3 +, CD4 +, CD8 +, CD19 +, HLA-DR +, and CD16 + / CD56 + levels were evaluated. In the statistical comparison of the groups, significance was only determined in respect of neutrophils. The results demonstrated the positive systematic and cellular effects of MSCs application on critically ill COVID-19 patients in a versatile way. This effect plays an important role in curing and reducing mortality in critically ill patients.
Databáze: OpenAIRE
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