Organization of mitochondrial DNA in the basidiomycetousDioszegia hungarica(Cryptococcus hungaricus)species
| Autor: | Attila Gácser, Ilona Pfeiffer, Judit Litter, Ferenc Kevei, Judit Kucsera, Zsuzsanna Hamari |
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| Rok vydání: | 2002 |
| Předmět: |
Genetics
Mitochondrial DNA Strain (biology) Restriction Mapping Biology DNA Mitochondrial Microbiology Yeast SmaI Fungal Proteins Cryptococcus Microscopy Electron Restriction enzyme Restriction site Restriction fragment length polymorphism DNA Fungal Molecular Biology Gene Polymorphism Restriction Fragment Length |
| Zdroj: | FEMS Microbiology Letters. 212:1-6 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2002.tb11236.x |
| Popis: | The organization of mitochondrial DNA was investigated in the six collection strains of the basidiomycetous yeast Dioszegia hungarica (Cryptococcus hungaricus) isolated so far. Physical and partial functional maps were constructed. Two strains (CBS 6324 and 6576) were identical while three others (CBS 4214, 5124, 6953) differed not only in the distribution of restriction sites but in gene order as well. Results confirm the hypothesis that these five strains are representatives of different closely related species. The sixth strain CBS 6569 revealed a unique mitochondrial genome organization. Its mtDNA separated into eight bands on agarose gel without enzymatic digestion. These molecules carried mitochondrial genes, and RFLP analysis of the four largest molecules using frequently-cutting restriction enzymes (KpnI and SmaI) showed them to have strongly homologous sequences. This unique mtDNA organization was also observed in a strain of Cystofilobasidium capitatum, providing evidence that CBS 6569 belongs to the Cystofilobasidium clade. |
| Databáze: | OpenAIRE |
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