Viability and Osteogenic Potential of Cryopreserved Human Bone Marrow-Derived Mesenchymal Cells
| Autor: | Kaori Muraki, Noriko Kotobuki, Yoshinori Takakura, Hiroko Machida, Youichi Katou, Motohiro Hirose, Hajime Ohgushi |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cryopreservation
Cell Survival Chemistry Cellular differentiation Mesenchymal stem cell Cell Culture Techniques General Engineering CD34 Bone Marrow Cells Cell Differentiation Mesenchymal Stem Cells Alkaline Phosphatase Flow Cytometry Fluoresceins Ascorbic acid Andrology medicine.anatomical_structure Osteogenesis Cell culture medicine Humans Bone marrow Bone regeneration Cell Proliferation |
| Zdroj: | Tissue Engineering. 11:663-673 |
| ISSN: | 1557-8690 1076-3279 |
| DOI: | 10.1089/ten.2005.11.663 |
| Popis: | Human bone marrow-derived mesenchymal cells contain mesenchymal stem cells (MSCs), which are well known for their osteo/chondrogenic potential and can be used for bone reconstruction. This article reports the viability of cryopreserved human mesenchymal cells and a comparison of the osteogenic potential between noncryopreserved and cryopreserved human mesenchymal cells with MSC-like characteristics, derived from the bone marrow of 28 subjects. The viability of cryopreserved mesenchymal cells was approximately 90% regardless of the storage term (0.3 to 37 months). It is clear by fluorescence-activated cell sorter analysis that the cell surface antigens of both noncryopreserved and cryopreserved mesenchymal cells were negative for hematopoietic cell markers such as CD14, CD34, CD45, and HLA-DR but positive for mesenchymal characteristics such as CD29 and CD105. To monitor the osteogenic potential of the cells, such as alkaline phosphatase (ALP) activity and in vitro mineralization, a subculture was conducted in the presence of dexamethasone, ascorbic acid, and glycerophosphate. No difference in osteogenic potential was found between cells with or without cryopreservation treatment. In addition, cells undergoing long-term cryopreservation (about 3 years) maintained high osteogenic potential. In conclusion, cryopreserved as well as noncryopreserved human mesenchymal cells could be applied for bone regeneration in orthopedics. |
| Databáze: | OpenAIRE |
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