Rat thymocytes release a factor which inhibits muscarinic ligand binding
| Autor: | Jan Ryzewski, Wlodzimierz Maslinski, Tamas Bartfai |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
medicine.medical_specialty Immunology Thymus Gland Internal medicine Muscarinic acetylcholine receptor medicine Immunology and Allergy Animals Incubation Chemistry Muscarinic antagonist Rats Inbred Strains Ligand (biochemistry) Trypsin Molecular biology Receptors Muscarinic Rats Quinuclidinyl Benzilate Thymus Hormones Thymocyte Membrane medicine.anatomical_structure Endocrinology Neurology Cerebral cortex Neurology (clinical) medicine.drug |
| Zdroj: | Journal of neuroimmunology. 17(4) |
| ISSN: | 0165-5728 |
| Popis: | Rat thymocytes incubated in RPMI medium for 120–300 min release a soluble factor of molecular weight below 10 000 Da which inhibits the binding of 3 H-labeled muscarinic antagonists in an uncompetitive manner, i.e. it reduces maximal specific binding ( B max) without changing the affinity of the ligand ( K D) . This factor inhibited muscarinic antagonist binding on thymocytes and rat cerebral cortex cellular membranes. Thymocytes from hydrocortisone-treated rats produced more factor per mg cell protein than thymocytes from untreated rats. The activity of the factor was unaffected by incubation with Cd 2+ (1 mM) or Zn 2+ (1 mM) or EDTA (1 mM), and its protein nature is supported by the following findings: it was trypsin sensitive, heat denaturated at 56°C and precipitated by (NH 4 ) 2 SO 4 (40%, w/v). Inhibition by the factor was apparently irreversible after 1 h of incubation. |
| Databáze: | OpenAIRE |
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