The catalase and superoxide dismutase genes are transcriptionally up-regulated upon oxidative stress in the strictly anaerobic archaeon Methanosarcina barkeri
| Autor: | Alexander I. Netrusov, Andrei L. Brioukhanov, Rik I.L. Eggen |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Paraquat
ved/biology.organism_classification_rank.species Oxidative phosphorylation medicine.disease_cause Microbiology Superoxide dismutase chemistry.chemical_compound medicine Anaerobiosis Hydrogen peroxide biology Superoxide Dismutase ved/biology Superoxide Hydrogen Peroxide Catalase Oxidants Up-Regulation Oxidative Stress chemistry Biochemistry biology.protein Methanosarcina barkeri Gene Expression Regulation Archaeal Oxidative stress |
| Zdroj: | Microbiology. 152:1671-1677 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.28542-0 |
| Popis: | Methanosarcina barkeriis a strictly anaerobic methanogenic archaeon, which can survive oxidative stress. The oxidative stress agent paraquat (PQ) suppressed growth ofM. barkeriat concentrations of 50–200 μM. Hydrogen peroxide (H2O2) inhibited growth at concentrations of 0.4–1.6 mM. Catalase activity in cell-free extracts ofM. barkeriincreased about threefold during H2O2stress (1.3 mM H2O2, 2–4 h exposure) and nearly twofold during superoxide stress (160 μM PQ, 2 h exposure). PQ (160 μM, 2–4 h exposure) and H2O2(1.3 mM, 2 h exposure) also influenced superoxide dismutase activity in cell-free extracts ofM. barkeri. Dot-blot analysis was performed on total RNA isolated from H2O2- and PQ-exposed cultures, using labelled internal DNA fragments of thesodandkatgenes. It was shown that H2O2but not PQ strongly induced up-regulation of thekatgene. PQ and to a lesser degree H2O2induced the expression of superoxide dismutase. The results indicate the regulation of the adaptive response ofM. barkerito different oxidative stresses. |
| Databáze: | OpenAIRE |
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