Transcripts of PTTG and Growth Factors bFGF and IGF-1 are Correlated in Pituitary Adenomas
| Autor: | Elmar Kirches, Knut Dietzmann, Kathrin Chamaon, Dimitrios Kanakis, C. Mawrin |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adenoma
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Basic fibroblast growth factor Biology Insulin-like growth factor chemistry.chemical_compound Endocrinology Downregulation and upregulation Pituitary adenoma Internal medicine Internal Medicine medicine Humans Pituitary Neoplasms RNA Messenger Insulin-Like Growth Factor I Regulation of gene expression Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Growth factor General Medicine medicine.disease Neoplasm Proteins Gene Expression Regulation Neoplastic Securin Real-time polymerase chain reaction chemistry Pituitary Gland Fibroblast Growth Factor 2 |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 118:121-126 |
| ISSN: | 1439-3646 0947-7349 |
| Popis: | The reasons for the increase of pituitary tumor-transforming gene (PTTG) transcripts in about 90% of pituitary adenomas are still not fully understood, although upregulation by basic fibroblast growth factor (bFGF) has been discussed as a potential cause. A possible influence of the Insulin like Growth Factor 1 (IGF-1) might be of interest, since this protein is also synthesized in most pituitary adenomas. Moreover, the principal regulation of the PTTG gene by IGF-1 and Insulin has been demonstrated in astrocytoma and breast cancer cells. We analyzed a large group (103 patients) of unselected clinical pituitary adenoma samples. From total RNA of frozen tumor samples (all subtypes) cDNA ( COMPLEMENTARY DNA) was synthesized and transcripts of PTTG, bFGF, IGF-1 were measured by Real-Time-PCR. Not only mRNA ( MESSENGER RNA) levels of bFGF, but also of IGF-1, correlated strongly with PTTG transcripts. This result was obtained, when all pituitary adenoma samples were included in the statistical calculations, irrespective of their subclassification. Our study suggests a connection between PTTG and IGF-1 in pituitary adenomas. |
| Databáze: | OpenAIRE |
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