Comparison of plasma from healthy nonsmokers, smokers, and lung cancer patients: Pattern-based differentiation profiling of low molecular weight proteins and peptides by magnetic bead technology with MALDI-TOF MS
| Autor: | Naghma Hashmi, Nadeem Rizvi, Ahmed Usman, Atta-ur-Rahman, M. Iqbal Choudhary, Syed Ghulam Musharraf |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Magnetic Bead Technology Lung Neoplasms Chromatography Chemistry Proteomic Profiling Health Toxicology and Mutagenesis Smoking Clinical Biochemistry Peptide Fractionation medicine.disease Mass spectrometry Biochemistry Neoplasm Proteins Matrix-assisted laser desorption/ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Magnetic bead Biomarkers Tumor medicine Humans Electrophoresis Polyacrylamide Gel Lung cancer Chromatography High Pressure Liquid |
| Zdroj: | Biomarkers. 17:223-230 |
| ISSN: | 1366-5804 1354-750X |
| DOI: | 10.3109/1354750x.2012.657245 |
| Popis: | Context: Smoking is the major contributor of lung cancer (LC), which accounts for millions of death.Objective: This study focused on the correlation between the proteomic profiling of LC patients, and healthy nonsmokers and smokers.Method: Pattern-based peptide profiling of 186 plasma samples was performed through reversed-phase chromatography-18 magnetic bead fractionation coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and resulted data were evaluated statistically by ClinProTool.Results: Marker peaks at m/z 1760, 5773, 5851, 2940, and 7172 were found with an excellent statistical figure.Conclusion: Selected marker peaks can be served as a differentiated tool of LC patients with high sensitivity and specificity. |
| Databáze: | OpenAIRE |
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