Identifying inhibitory effects of lignocellulosic by-products on growth of lactic acid producing micro-organisms using a rapid small-scale screening method
| Autor: | Edwin C. van der Pol, Gerrit Eggink, Ruud A. Weusthuis, E.M.J. Vaessen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Bio Process Engineering Environmental Engineering Formic acid Bioengineering Alkalies Bacterial growth Furfural Coumaric acid Lignin 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Acetic acid 010608 biotechnology Lactobacillus Anaerobiosis Food science Waste Management and Disposal Food Process Engineering VLAG Inhibition biology Bacteria Renewable Energy Sustainability and the Environment Lactic acid General Medicine biology.organism_classification Lignocellulosic by-products 030104 developmental biology BBP Bioconversion chemistry Biochemistry Fermentation Screening Acids Biotechnology |
| Zdroj: | Bioresource Technology 209 (2016) Bioresource Technology, 209, 297-304 |
| ISSN: | 0960-8524 |
| Popis: | Sugars obtained from pretreated lignocellulose are interesting as substrate for the production of lactic acid in fermentation processes. However, by-products formed during pretreatment of lignocellulose can inhibit microbial growth. In this study, a small-scale rapid screening method was used to identify inhibitory effects of single and combined by-products on growth of lactic acid producing micro-organisms. The small-scale screening was performed in 48-well plates using 5 bacterial species and 12 by-products. Large differences were observed in inhibitory effects of by-products between different species. Predictions can be made for growth behaviour of different micro-organisms on acid pretreated or alkaline pretreated bagasse substrates using data from the small-scale screening. Both individual and combined inhibition effects were shown to be important parameters to predict growth. Synergy between coumaric acid, formic acid and acetic acid is a key inhibitory parameter in alkaline pretreated lignocellulose, while furfural is a key inhibitor in acid pretreated lignocellulose. |
| Databáze: | OpenAIRE |
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