An indirect enzyme-linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serovar 7 in pig serum
| Autor: | Lars Ekeroth, Lars Ole Andresen, Joan Klausen, Jan Grøndahl-Hansen |
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| Rok vydání: | 2007 |
| Předmět: |
0301 basic medicine
Serotype Lipopolysaccharides Lipopolysaccharide 040301 veterinary sciences Swine animal diseases Denmark 030106 microbiology Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Microbiology 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Reference test Actinobacillus Infections Antigen Animals Actinobacillus pleuropneumoniae chemistry.chemical_classification Swine Diseases General Veterinary biology Complement Fixation Tests 04 agricultural and veterinary sciences respiratory system biology.organism_classification Complement fixation test Virology Antibodies Bacterial Specific Pathogen-Free Organisms Enzyme chemistry biology.protein Antibody |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 19(3) |
| ISSN: | 1040-6387 |
| Popis: | Lipopolysaccharide (LPS) antigen was purified from Actinobacillus pleuropneumoniae serovar 7 by phenol-water extraction and fractionated on a, S-100 Sephacryl column. High molecular weight fractions of LPS purified from the S-100 column were pooled and used as antigen in an indirect serovar 7 ELISA. The ELISA was evaluated with sera from pigs experimentally infected with 11 different A. pleuropneumoniae serovars of biotype 1. Estimation of sensitivity and specificity of the A. pleuropneumoniae serovar 7 ELISA was performed using pig sera from herds naturally infected with A. pleuropneumoniae serovar 7 as well as sera from herds free of infection with A. pleuropneumoniae serovar 7. When compared to the complement fixation test (CFT) as a reference test, the ELISA showed much higher sensitivity and statistically equivalent specificity. |
| Databáze: | OpenAIRE |
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