Circulating Tumour Cell Numbers Correlate with Platelet Count and Circulating Lymphocyte Subsets in Men with Advanced Prostate Cancer: Data from the ExPeCT Clinical Trial (CTRIAL-IE 15-21)
| Autor: | Dearbhaile M. O'Donnell, Juliette Hussey, Jean Dunne, Thomas R. Lynch, Stephen P. Finn, Brian D. Hayes, John Greene, Nicola Peat, John J. O'Leary, Emma H. Allott, Rustom P. Manecksha, Sarah Rudman, Moya Cunningham, Orla Casey, Grainne Sheill, Lauren Brady, Emer Guinan, Liam Grogan, John McCaffrey, Mieke Van Hemelrijck, Anne-Marie Baird, Tatjana Vlajnic, Bryan Stanfill, Fidelma Cahill, Lorelei A. Mucci, Ray McDermott, Orla Sheils, Dean Holden, Verena Murphy |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
medicine.medical_specialty obesity Randomization circulating tumour cells Lymphocyte Inflammation Overweight Gastroenterology Article Flow cytometry Metastasis Prostate cancer SDG 3 - Good Health and Well-being Internal medicine medicine Platelet coagulation RC254-282 medicine.diagnostic_test exercise business.industry flow cytometry Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease prostate cancer medicine.anatomical_structure Oncology inflammation platelets medicine.symptom business |
| Zdroj: | Cancers Hayes, B, Brady, L, Sheill, G, Baird, A-M, Guinan, E, Stanfill, B, Dunne, J, Holden, D, Vlajnic, T, Casey, O, Murphy, V, Greene, J, Allott, E H, Hussey, J, Cahill, F, Hemelrijck, M V, Peat, N, Mucci, L A, Cunningham, M, Grogan, L, Lynch, T, Manecksha, R P, McCaffrey, J, O’Donnell, D M, Sheils, O, O’Leary, J J, Rudman, S, McDermott, R & Finn, S 2021, ' Circulating Tumour Cell Numbers Correlate with Platelet Count and Circulating Lymphocyte Subsets in Men with Advanced Prostate Cancer: Data from the ExPeCT Clinical Trial (CTRIAL-IE 15-21) ', Cancers, vol. 13, no. 18, 4690 . https://doi.org/10.3390/cancers13184690 Cancers, Vol 13, Iss 4690, p 4690 (2021) Volume 13 Issue 18 |
| ISSN: | 2072-6694 |
| DOI: | 10.3390/cancers13184690 |
| Popis: | Interactions between circulating tumour cells (CTCs) and platelets are thought to inhibit natural killer(NK)-cell-induced lysis. We attempted to correlate CTC numbers in men with advanced prostate cancer with platelet counts and circulating lymphocyte numbers. Sixty-one ExPeCT trial participants, divided into overweight/obese and normal weight groups on the basis of a BMI ≥ 25 or < 25, were randomized to participate or not in a six-month exercise programme. Blood samples at randomization, and at three and six months, were subjected to ScreenCell filtration, circulating platelet counts were obtained, and flow cytometry was performed on a subset of samples (n = 29). CTC count positively correlated with absolute total lymphocyte count (r2 = 0.1709, p = 0.0258) and NK-cell count (r2 = 0.49, p < 0.0001). There was also a positive correlation between platelet count and CTC count (r2 = 0.094, p = 0.0001). Correlation was also demonstrated within the overweight/obese group (n = 123, p < 0.0001), the non-exercise group (n = 79, p = 0.001) and blood draw samples lacking platelet cloaking (n = 128, p < 0.0001). By flow cytometry, blood samples from the exercise group (n = 15) had a higher proportion of CD3+ T-lymphocytes (p = 0.0003) and lower proportions of B-lymphocytes (p = 0.0264) and NK-cells (p = 0.015) than the non-exercise group (n = 14). These findings suggest that CTCs engage in complex interactions with the coagulation cascade and innate immune system during intravascular transit, and they present an attractive target for directed therapy at a vulnerable stage in metastasis. |
| Databáze: | OpenAIRE |
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