Phosphorylation and Alternative Translation on Wheat Germ Cell-Free Protein Synthesis of the DHBV Large Envelope Protein
| Autor: | Anja Böckmann, Adeline Page, Guillaume David, Marie-Laure Fogeron, Lauriane Lecoq, Roland Montserret, Frédéric Delolme, Michael Nassal |
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| Přispěvatelé: | Microbiologie moléculaire et biochimie structurale / Molecular Microbiology and Structural Biochemistry (MMSB), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), SFR Biosciences, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Freiburg [Freiburg], Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Bockmann, Anja, École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Duck hepatitis B virus Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry alternative translation initiation 03 medical and health sciences 0302 clinical medicine Eukaryotic translation cell-free protein synthesis (CFPS) wheat-germ Protein biosynthesis Molecular Biosciences [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] lcsh:QH301-705.5 [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology Cell-free protein synthesis phosporylation biology Chemistry Translation (biology) Brief Research Report Viral membrane biology.organism_classification Transmembrane protein [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] 3. Good health Cell biology 030104 developmental biology lcsh:Biology (General) HBsAg—surface antigen of hepatitis B virus 030220 oncology & carcinogenesis HBsAg-surface antigen of hepatitis B virus [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Phosphorylation |
| Zdroj: | Frontiers in Molecular Biosciences Frontiers in Molecular Biosciences, Frontiers Media, 2019, 6, pp.138. ⟨10.3389/fmolb.2019.00138⟩ Frontiers in Molecular Biosciences, 2019, 6, pp.138. ⟨10.3389/fmolb.2019.00138⟩ Frontiers in Molecular Biosciences, Vol 6 (2019) |
| ISSN: | 2296-889X |
| DOI: | 10.3389/fmolb.2019.00138 |
| Popis: | International audience; Wheat-germ cell-free protein synthesis (WG-CFPS) is a potent platform for the high-yield production of proteins. It is especially of interest for difficult-to-express eukaryotic proteins, such as toxic and transmembrane proteins, and presents an important tool in high-throughput protein screening. Until recently, an assumed drawback of WG-CFPS was a reduced capacity for post-translational modifications. Meanwhile, phosphorylation has been observed in WG-CFPS; yet, authenticity of the respective phosphorylation sites remained unclear. Here we show that a viral membrane protein, the duck hepatitis B virus (DHBV) large envelope protein (DHBs L), produced by WG-CFPS, is phosphorylated upon translation at the same sites as DHBs L produced during DHBV infection of primary hepatocytes. Furthermore, we show that alternative translation initiation of the L protein, previously identified in virus-producing hepatic cells, occurs on WG-CFPS as well. Together, these findings further strengthen the high potential of WG-CFPS to include the reproduction of specific modifications proteins experience in vivo. |
| Databáze: | OpenAIRE |
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