Galectin-9 Activates and Expands Human T-Helper 1 Cells
| Autor: | Paul Eggleton, Valerie R. Wiersma, Toshiro Niki, Marloes J. M. Gooden, Hans W. Nijman, Edwin Bremer, Mitsuomi Hirashima, Jurjen Gerssen, Wijnand Helfrich, Robert J. van Ginkel, Douwe F. Samplonius |
|---|---|
| Přispěvatelé: | Targeted Gynaecologic Oncology (TARGON), Translational Immunology Groningen (TRIGR), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Stem Cell Aging Leukemia and Lymphoma (SALL) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
Glycobiology lcsh:Medicine Lymphocyte Activation Biochemistry 0302 clinical medicine Molecular Cell Biology INFECTION ANTITUMOR IMMUNITY Cytotoxic T cell SUPPRESSES Receptor lcsh:Science Hepatitis A Virus Cellular Receptor 2 Immune Response 0303 health sciences Multidisciplinary Cell Death T Cells T-Lymphocytes Helper-Inducer Signaling in Selected Disciplines Cell biology APOPTOSIS Phenotype 030220 oncology & carcinogenesis Medicine ARTHRITIS Research Article Signal Transduction Programmed cell death Galectins Immune Cells TIM-3 Immunology Receptors Antigen T-Cell Biology Immunological Signaling DENDRITIC CELLS Immunomodulation 03 medical and health sciences Humans REGULATORY T-CELLS 030304 developmental biology Galectin Dose-Response Relationship Drug T-cell receptor lcsh:R Membrane Proteins BINDING LECTIN MICE Apoptosis Leukocytes Mononuclear Clinical Immunology lcsh:Q Immunologic Memory Ex vivo CD8 |
| Zdroj: | PLoS ONE, Vol 8, Iss 5, p e65616 (2013) PLoS ONE, 8(5):e65616. PUBLIC LIBRARY SCIENCE PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0065616 |
| Popis: | Galectin-9 (Gal-9) is known for induction of apoptosis in IFN-gamma and IL-17 producing T-cells and amelioration of autoimmunity in murine models. On the other hand, Gal-9 induced IFN-gamma positive T-cells in a sarcoma mouse model and in food allergy, suggesting that Gal-9 can have diametric effects on T-cell immunity. Here, we aimed to delineate the immunomodulatory effect of Gal-9 on human resting and ex vivo activated peripheral blood lymphocytes. Treatment of resting lymphocytes with low concentrations of Gal-9 (5-30 nM) induced apoptosis in similar to 60% of T-cells after 1 day, but activated the surviving T-cells. These viable T-cells started to expand after 4 days with up to 6 cell divisions by day 7 and an associated shift from naive towards central memory and IFN-gamma producing phenotype. In the presence of T-cell activation signals (anti-CD3/IL-2) Gal-9 did not induce T-cell expansion, but shifted the CD4/CD8 balance towards a CD4-dominated T-cell response. Thus, Gal-9 activates resting T-cells in the absence of typical T-cell activating signals and promotes their transition to a T-H1/C1 phenotype. In the presence of T-cell activating signals T-cell immunity is directed towards a CD4-driven response by Gal-9. Thus, Gal-9 may specifically enhance reactive immunological memory. |
| Databáze: | OpenAIRE |
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