Soluble N-cadherin fragment promotes angiogenesis
| Autor: | O. De Wever, L. Morbidelli, E Van Aken, Lara Derycke, Marc Bracke, M. Ziche |
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| Rok vydání: | 2006 |
| Předmět: |
Angiogenesis
Endothelial cells Fibroblast growth factor receptor Migration N-cadherin Cancer Research Time Factors Neovascularization Physiologic Biology Fibroblast growth factor Vascular endothelial growth inhibitor Models Biological Cell Line Cornea Vasculogenesis Cell Movement Medicine and Health Sciences Animals Humans Phosphorylation Wound Healing General Medicine Cadherins Receptors Fibroblast Growth Factor Molecular biology Cell biology Vascular endothelial growth factor B Vascular endothelial growth factor A Oncology Vascular endothelial growth factor C Endothelium Vascular Rabbits Wound healing |
| Zdroj: | CLINICAL & EXPERIMENTAL METASTASIS |
| ISSN: | 1573-7276 0262-0898 |
| DOI: | 10.1007/s10585-006-9029-7 |
| Popis: | Endothelial cells express two dependent intercellular adhesion molecules: vascular endothelial (VE)-cadherin, specific for endothelial cells, and N-cadherin, also present in neuronal, lens, skeletal and heart muscle cells, osteoblasts, pericytes and fibroblasts. While there exists a vast amount of evidence that VE-cadherin promotes angiogenesis, the role of N-cadherin still remains to be elucidated. We found that a soluble 90-kDa fragment N-cadherin promotes angiogenesis in the rabbit cornea assay and in the chorioallantoic assay when cleaved enzymatically from the extracellular domain of N-cadherin. Soluble N-cadherin stimulates migration of endothelial cells in the wound healing assay and stimulates phosphorylation of extracellular regulated kinase. In vitro experiments with PD173074 and knock-down of N-cadherin and fibroblast growth factor (FGF)-receptor, showed that the pro-angiogenic effect of soluble N-cadherin is N-cadherin- and FGF-receptor-dependent. Our results suggest that soluble N-cadherin stimulates migration of endothelial cells through the FGF-receptor. |
| Databáze: | OpenAIRE |
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