Panton-Valentine leukocidin and staphylococcal cassette chromosome mec characterization of community acquired methicillin-resistant Staphylococcus aureus
| Autor: | Damir Rebić, Velma Rebic, Ana Budimir, Sabina Mahmutovic Vranic, Mufida Aljicevic |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Bacterial Toxins Exotoxins Microbial Sensitivity Tests Biology medicine.disease_cause Chromosomes Microbiology 03 medical and health sciences chemistry.chemical_compound Methicillin 0302 clinical medicine Antibiotic resistance Bacterial Proteins Leukocidins medicine Pulsed-field gel electrophoresis Humans Penicillin-Binding Proteins 030212 general & internal medicine Bosnia and Herzegovina 0303 health sciences SCCmec Public Health Environmental and Occupational Health 030311 toxicology General Medicine biochemical phenomena metabolism and nutrition Staphylococcal Infections bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents Community-Acquired Infections chemistry Staphylococcus aureus Linezolid Vancomycin Panton–Valentine leukocidin medicine.drug |
| Zdroj: | Central European journal of public health. 27(1) |
| ISSN: | 1210-7778 |
| Popis: | Objectives: Staphylococcus aureus (SA) represents one of the most important microorganism that is part of the normal microflora of humans, but in certain conditions can cause very serious infections. Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for a wide spectrum of nosocomial and community associated infections worldwide. The aim of this study was to determine community acquired MRSA (CA-MRSA), as well as the frequency of Panton-Valentine leukocidin (PVL) genes and staphylococcal cassette chromosome mec (SCCmec) types in isolates obtained from outpatients in the region of 700,000 people (Canton Sarajevo, Bosnia and Herzegovina) Methods: Our investigation included phenotypic and genotypic markers such as antimicrobial resistance, pulsed-field gel electrophoresis (PFGE), SCC typing, and PVL detection. Results: Antimicrobial susceptibility: all MRSA isolates were resistant to the β-lactam antibiotics tested, and all isolates were susceptible to trimethoprim sulphamethoxazole, rifampicin, fusidic acid, linezolid, and vancomycin. After the PFGE analysis, the isolates were grouped into five similarity groups: A-E. The largest number of isolates belonged to one of two groups: C - 60% and D - 27%. In both groups C and D, SCCmec type IV was predominant (60% and 88.8%, respectively). A total of 24% of the isolates had positive expression of PVL genes, while 76% showed a statistically significantly greater negative expression of PVL genes. Conclusions: Using combination techniques, we were able to investigate the origin and genetic background of the strains. PFGE analysis revealed two large, genetically related groups of strains consisting of 87 isolates. Our results suggest failure to apply the screening policy, and a lack of knowledge about multiresistant MRSA strains. This study showed the local epidemiological situation which should be the basis of antimicrobial empiric therapy for non-hospitalized patients. |
| Databáze: | OpenAIRE |
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