E-cadherin is regulated by GATA-2 and marks the early commitment of mouse hematopoietic progenitors to the basophil and mast cell fates
| Autor: | Hajime Karasuyama, Nouraiz Ahmed, Kensuke Miyake, Francisco Marchi, Shin-Young Park, Haoran Zhang, Anuya Paranjape, Marta Borchiellini, Stephen J. Galli, Sisi Chen, Junyan Zhang, Daniel G. Tenen, Elaine Dzierzak, Annalisa Di Ruscio, Sweta B. Patel, Samanta A. Mariani, Timm Schroeder, Mindy Tsai, Mahmoud A. Bassal, Robert S. Welner, Anais Wanet |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Primary Cell Culture Immunology Cell Priming (immunology) Biology Basophil Mice 03 medical and health sciences 0302 clinical medicine Immunophenotyping medicine Animals Cell Lineage Mast Cells RNA-Seq Progenitor cell Cells Cultured Cadherin Cell Differentiation hemic and immune systems General Medicine Cadherins Hematopoietic Stem Cells Mast cell Basophils Cell biology GATA2 Transcription Factor Haematopoiesis 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Single-Cell Analysis |
| Zdroj: | Science Immunology, 6 (56) |
| ISSN: | 2470-9468 |
| Popis: | E-cadherin is a calcium-dependent cell-cell adhesion molecule extensively studied for its involvement in tissue formation, epithelial cell behavior, and suppression of cancer. However, E-cadherin expression in the hematopoietic system has not been fully elucidated. Combining single-cell RNA-sequencing analyses and immunophenotyping, we revealed that progenitors expressing high levels of E-cadherin and contained within the granulocyte-monocyte progenitors (GMPs) fraction have an enriched capacity to differentiate into basophils and mast cells. We detected E-cadherin expression on committed progenitors before the expression of other reported markers of these lineages. We named such progenitors pro-BMPs (pro-basophil and mast cell progenitors). Using RNA sequencing, we observed transcriptional priming of pro-BMPs to the basophil and mast cell lineages. We also showed that GATA-2 directly regulates E-cadherin expression in the basophil and mast cell lineages, thus providing a mechanistic connection between the expression of this cell surface marker and the basophil and mast cell fate specification. ISSN:2470-9468 |
| Databáze: | OpenAIRE |
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