Characterization of the Flavonoid-Responsive Regulator FrrA and Its Binding Sites
| Autor: | Mandy Wenzel, Bianca Kranzusch, Anita Bhandari, Tobias Günther, Michael Göttfert, Pavel Lulchev, Andy Weiss, Kathrin Lang, Erik Szentgyörgyi |
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| Rok vydání: | 2012 |
| Předmět: |
Flavonoids
Operator (biology) Operon Daidzein food and beverages Codon Initiator Gene Expression Regulation Bacterial Articles Biology Plant Root Nodulation Microbiology Molecular biology Response regulator chemistry.chemical_compound Bacterial Proteins Start codon chemistry Mutation TetR Bradyrhizobium Soybeans Binding site Molecular Biology Gene |
| Zdroj: | Journal of Bacteriology. 194:2363-2370 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Previous microarray analyses revealed that in Bradyrhizobium japonicum , about 100 genes are induced by genistein, an isoflavonoid secreted by soybean. This includes the three genes freC , freA , and freB (systematic designations bll4319 , bll4320 , and bll4321 ), which are likely to form a genistein-, daidzein-, and coumestrol-inducible operon and to encode a multidrug efflux system. Upstream of freCAB and in the opposite orientation, FrrA (systematic designation Blr4322), which has similarity to TetR-type regulators, is encoded. A deletion of frrA leads to increased expression of freB in the absence of an inducer. We identified the correct translational start codon of frrA and showed that the gene is inducible by genistein and daidzein. The protein, which was heterologously expressed and purified from Escherichia coli , binds to two palindrome-like DNA elements (operator A and operator B), which are located in the intergenic region between freC and frrA . The replacement of several nucleotides or the insertion of additional spacer nucleotides prevented binding. Binding of FrrA was also affected by the addition of genistein. By mapping the transcription start sites, we found that operator A covers the transcriptional start site of freC and operator B is probably located between the −35 regions of the two divergently oriented genes. Operator A seems to be conserved in a few similar gene constellations in other proteobacteria. Our data indicate that in B. japonicum , besides NodD1 (the LysR family) and NodVW (a two-component response regulator), a third regulator type (a TetR family member) which responds to the plant signal molecules genistein and daidzein exists. |
| Databáze: | OpenAIRE |
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