Characteristics of a Pink-Pigmented Bacterium Isolated from Biofilm in a Cooling Tower in Tokyo, Japan
| Autor: | Keiko Saitou, Keiichi Goto, Motonobu Hara, Shin-ichi Yoshida, Jun-Ichi Sugiyama, Yuko Kato, Katsunori Furuhata, Masafumi Fukuyama |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Immunology Microbial Sensitivity Tests Polymerase Chain Reaction Microbiology chemistry.chemical_compound Pigment RNA Ribosomal 16S Virology Tokyo Phylogeny Base Composition Base Sequence Phylogenetic tree biology Strain (chemistry) Pigmentation Fatty Acids Biofilm biology.organism_classification 16S ribosomal RNA Roseomonas gilardii chemistry Biofilms visual_art visual_art.visual_art_medium Malic acid Water Microbiology Methylobacteriaceae Sequence Alignment Bacteria |
| Zdroj: | Microbiology and Immunology. 51:637-641 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.2007.tb03951.x |
| Popis: | Strain K-20, a Gram-negative, non-motile, non-spore-forming and strictly aerobic rod, which produces a pale pink pigment, was isolated from biofilm in a cooling tower in Tokyo, Japan. The taxonomic feature of the strain was studied using phenotypic tests and phylogenetic analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was related to Roseomonas gilardii subsp. rosea, Roseomonas gilardii subsp. gilardii, Roseomonas cervicalis and Roseomonas mucosa at 94.3-94.6 sequence similarities. Growth occurred at 25-40 C and pH 5.0-10.0, optimal at 35 C and pH 7.0. Growth did not occur in the presence ofor=2% NaCl. The API 20NE identification system gave a positive result for urease, L-arabinose, potassium gluconate, adipic acid, malic acid and trisodium citrate (API code number 0201465). The predominant fatty acids of strain K-20 were C18:1Delta11 (50.8%) and C16:1 (17.2%). Cells contained ubiquinone 10 (Q-10) as the major quinone and the G+C content was 72.0 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data, it was assumed that strain K-20 (=JCM 14634) is a novel species of the genus Roseomonas. |
| Databáze: | OpenAIRE |
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