Factor XII gene expression in endometrial stromal cells during decidualisation
| Autor: | Nao Murabayashi, Dong Fang Wang, Hiroaki Kawato, Ning Ma, Tsutomu Tabata, Norimasa Sagawa, Hiroyuki Minoura |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Stromal cell Time Factors Reproductive immunology Blotting Western Reproductive technology Biology Endometrium Andrology Endocrinology Pregnancy Gene expression Genetics medicine Decidua Humans Embryo Implantation RNA Messenger Molecular Biology reproductive and urinary physiology Cells Cultured Menstrual Cycle Progesterone Oligonucleotide Array Sequence Analysis Factor XII Estradiol urogenital system Microarray analysis techniques Gene Expression Profiling Gene Expression Regulation Developmental Immunohistochemistry Coculture Techniques Up-Regulation Blot Pregnancy Trimester First medicine.anatomical_structure Reproductive Medicine embryonic structures Immunology Animal Science and Zoology Female Chorionic Villi Stromal Cells Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 21(7) |
| ISSN: | 1031-3613 |
| Popis: | Decidualisation of endometrial stromal cells (ESC) is a prerequisite for the implantation of human embryos. Identification of genes that are upregulated or downregulated during decidualisation could lead to a better understanding of the molecular mechanisms involved in this process. In the present study, we examined differences in gene expression between decidualised and non-decidualised cells using microarray analysis and found that Factor XII (FXII) gene expression was upregulated during decidualisation. Furthermore, we also examined the expression of FXII by human ESC before and during pregnancy, as well as its expression by cells that had undergone decidualisation in vitro. Weak expression of FXII mRNA was detected in the non-pregnant endometrium that increased gradually from the proliferative to the secretory endometrium. During pregnancy, FXII mRNA expression was markedly increased in decidualised endometrium. When sex steroids (200 pg mL–1 of 17β-oestradiol and 100 ng mL–1 of progesterone) were used to induce in vitro decidualisation of ESC, the expression of FXII mRNA increased by approximately 25.3-fold compared with that in non-decidualised ESC. Using western blotting, we confirmed the presence of FXII protein (80 kDa) in ESC after in vitro decidualisation. Increased expression of FXII in ESC during decidualisation suggests that the kallikrein–kininogen–kinin system may be activated during the implantation of human embryos. |
| Databáze: | OpenAIRE |
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