Cell-specific expression of lung disease risk-related genes in the human small airway epithelium
Autor: | M.J. Thomas, Sarah L. O’Beirne, Yael Strulovici-Barel, Juergen Schymeinsky, Philip L. Leopold, Mahboubeh Rostami, Robert J. Kaner, Wu-Lin Zuo, Michelle G. LeBlanc, Karsten Quast, Jay S. Fine, Sudha Visvanathan, Ronald G. Crystal, Jacqueline Salit, Shushila A. Shenoy, Jason G. Mezey |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Lung Diseases Lung Neoplasms Cell Gene Expression Respiratory Mucosa Epithelial cells Cigarette Smoking Lung Disorder Pathogenesis Transcriptome 03 medical and health sciences Idiopathic pulmonary fibrosis Pulmonary Disease Chronic Obstructive 0302 clinical medicine Immune system Immune/inflammatory cells Bronchoscopy medicine Inherited and acquired pulmonary disorders Humans Single-cell transcriptomes Genetic Testing lcsh:RC705-779 Lung business.industry Sequence Analysis RNA Research lcsh:Diseases of the respiratory system medicine.disease 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Immunology Respiratory epithelium Airway Remodeling business |
Zdroj: | Respiratory Research Respiratory Research, Vol 21, Iss 1, Pp 1-11 (2020) |
ISSN: | 1465-993X |
Popis: | Background The human small airway epithelium (SAE) plays a central role in the early events in the pathogenesis of most inherited and acquired lung disorders. Little is known about the molecular phenotypes of the specific cell populations comprising the SAE in humans, and the contribution of SAE specific cell populations to the risk for lung diseases. Methods Drop-seq single-cell RNA-sequencing was used to characterize the transcriptome of single cells from human SAE of nonsmokers and smokers by bronchoscopic brushing. Results Eleven distinct cell populations were identified, including major and rare epithelial cells, and immune/inflammatory cells. There was cell type-specific expression of genes relevant to the risk of the inherited pulmonary disorders, genes associated with risk of chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis and (non-mutated) driver genes for lung cancers. Cigarette smoking significantly altered the cell type-specific transcriptomes and disease risk-related genes. Conclusions This data provides new insights into the possible contribution of specific lung cells to the pathogenesis of lung disorders. |
Databáze: | OpenAIRE |
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