Embelin downregulated cFLIP in breast cancer cell lines facilitate anti-tumor effect of IL-1β-stimulated human umbilical cord mesenchymal stem cells
| Autor: | Hwai Shi Wang, Eric Hung, Ya Han Liang, Jui Wen Teng, Jiann Ming Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell death Cell biology medicine.medical_treatment Science Interleukin-1beta CASP8 and FADD-Like Apoptosis Regulating Protein Down-Regulation Apoptosis Breast Neoplasms Mesenchymal Stem Cell Transplantation Article Targeted therapy Umbilical Cord 03 medical and health sciences 0302 clinical medicine Breast cancer medicine Benzoquinones Humans Cells Cultured Cancer Multidisciplinary business.industry Mesenchymal stem cell Drug Synergism Mesenchymal Stem Cells medicine.disease Coculture Techniques Radiation therapy 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Cancer research MCF-7 Cells Medicine Tumor necrosis factor alpha Female Drug Screening Assays Antitumor business Homing (hematopoietic) |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Breast cancer is the leading cause of cancer-related death for women. In breast cancer treatment, targeted therapy would be more effective and less harmful than radiotherapy or systemic chemotherapy. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been shown to induce apoptosis in cancer cells but not in normal cells. Mesenchymal stem cells have shown great therapeutic potential in cancer therapy owing to their ability of homing to tumor sites and secreting many kinds of anti-tumor proteins including TRAIL. In this study, we found that IL-1β-stimulated human umbilical cord-derived mesenchymal stem cells (hUCMSCs) enhance the expression of membrane-bound and soluble TRAIL. Cellular FADD-like IL-1β-converting enzyme inhibitory protein (cFLIP) is an important regulator in TRAIL-mediated apoptosis and relates to TRAIL resistance in cancer cells. Previous studies have shown that embelin, which is extracted from Embelia ribes, can increase the TRAIL sensitivity of cancer cells by reducing cFLIP expression. Here we have demonstrated that cFLIPL is correlated with TRAIL-resistance and that embelin effectively downregulates cFLIPL in breast cancer cells. Moreover, co-culture of IL-1β-stimulated hUCMSCs with embelin-treated breast cancer cells could effectively induce apoptosis in breast cancer cells. The combined effects of embelin and IL-1β-stimulated hUCMSCs may provide a new therapeutic strategy for breast cancer therapy. |
| Databáze: | OpenAIRE |
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