Analysis of the tet gene of plasmid pCIS7 isolated from Bacillus subtilis
| Autor: | Catherine L. Ives, Kenneth F. Bott |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial Bacillaceae biology R Factors Molecular Sequence Data Nucleic acid sequence Tetracycline Resistance General Medicine Bacillus subtilis Molecular cloning biology.organism_classification Molecular biology Repressor Proteins chemistry.chemical_compound Plasmid chemistry Genes Bacterial Sequence Homology Nucleic Acid Gene expression Genetics Amino Acid Sequence Gene DNA |
| Zdroj: | Gene. 94(1) |
| ISSN: | 0378-1119 |
| Popis: | We have previously shown that plasmid pCIS7, which contains 11.5 kb of Bacillus subtilis DNA isolated from a tetracycline-sensitive (Tc S ) strain, confers Tc resistance when integrated and amplified in the chromosome of Tc S B. subtilis 168 trp C2 [Ives and Bott, J. Bacteriol. 171 (1989) 1801–1810]. Here, we report that the number of integrated plasmid sequences required to confer Tc resistance is greater than the 20 copies seen with increasing chloramphenicol selection and, by dot-blot analysis, exceeds 100 copies per cell. The amplification is accompanied by a corresponding increase in mRNA encoding the tet gene. The tet gene sequence of pCIS7 has been compared to B. subtilis tet GSY908 [Sakaguchi et al., Biochim. Biophys. Acta. 94 (1988) 49–57] and other Gram-positive tet genes. The tet gene of pCIS7 is a member of the class L Tc R determinants, and probably confers Tc resistance by increasing the efflux of Tc from the bacterial cell. |
| Databáze: | OpenAIRE |
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