Phosphite, an Analog of Phosphate, Suppresses the Coordinated Expression of Genes under Phosphate Starvation
| Autor: | Deepa K. Varadarajan, Paino Durzo Matilda, Kashchandra G. Raghothama, Athikkattuvalasu S. Karthikeyan |
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| Rok vydání: | 2002 |
| Předmět: |
Phosphites
Transcription Genetic Physiology Recombinant Fusion Proteins Saccharomyces cerevisiae Arabidopsis Pancreatitis-Associated Proteins Plant Science Plant Roots Phosphates Solanum lycopersicum Gene Expression Regulation Plant Gene expression Genetics Pi Phosphate Transport Proteins Luciferases Gene Plant Proteins Regulation of gene expression biology Acid phosphatase Plants Genetically Modified biology.organism_classification Biochemistry biology.protein Signal transduction Starvation response Plant Shoots Research Article |
| Zdroj: | Plant Physiology. 129:1232-1240 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.010835 |
| Popis: | Phosphate (Pi) and its analog phosphite (Phi) are acquired by plants via Pi transporters. Although the uptake and mobility of Phi and Pi are similar, there is no evidence suggesting that plants can utilize Phi as a sole source of phosphorus. Phi is also known to interfere with many of the Pi starvation responses in plants and yeast (Saccharomyces cerevisiae). In this study, effects of Phi on plant growth and coordinated expression of genes induced by Pi starvation were analyzed. Phi suppressed many of the Pi starvation responses that are commonly observed in plants. Enhanced root growth and root to shoot ratio, a hallmark of Pi stress response, was strongly inhibited by Phi. The negative effects of Phi were not obvious in plants supplemented with Pi. The expression of Pi starvation-induced genes such as LePT1, LePT2,AtPT1, and AtPT2 (high-affinity Pi transporters); LePS2 (a novel acid phosphatase);LePS3 and TPSI1 (novel genes); andPAP1 (purple acid phosphatase) was suppressed by Phi in plants and cell cultures. Expression of luciferase reporter gene driven by the Pi starvation-induced AtPT2 promoter was also suppressed by Phi. These analyses showed that suppression of Pi starvation-induced genes is an early response to addition of Phi. These data also provide evidence that Phi interferes with gene expression at the level of transcription. Synchronized suppression of multiple Pi starvation-induced genes by Phi points to its action on the early molecular events, probably signal transduction, in Pi starvation response. |
| Databáze: | OpenAIRE |
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