Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF)
| Autor: | Mauricio Langleib, Mariana Andrea Papalia, Gabriel Osvaldo Gutkind, Marcela Alejandra Radice, Carla Steffanowski, Maria Soledad Ramirez, Andrés Iriarte, Laura Galanternik, Vaughn S. Cooper |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Achromobacter Cystic Fibrosis Achromobacter ruhlandii Integron medicine.disease_cause Microbiology 03 medical and health sciences Plasmid medicine Humans Child 0303 health sciences biology 030306 microbiology Pseudomonas aeruginosa General Medicine Achromobacter xylosoxidans biology.organism_classification biology.protein Mobile genetic elements Antitoxin Gram-Negative Bacterial Infections Plasmids |
| Zdroj: | Revista Argentina de microbiologia. 54(1) |
| ISSN: | 0325-7541 |
| Popis: | In the last decade Achromobacter spp. has been associated with chronic colonization in patients with cystic fibrosis (CF). Although Achromobacter xylosoxidans is the most frequent species recovered within this genus, other species such as A. ruhlandii have also been reported in these patients. Descriptions of mobile elements are scarce in Achromobacter and none of them have been originated in A. ruhlandii. The aim of this study was to report the full characterization of a plasmid which was maintained in four clonally related A. ruhlandii isolates. Between 2013 and 2015, nine A. ruhlandii isolates were recovered from a pediatric patient with CF at a hospital in Buenos Aires. Four selected clonally related isolates were sequenced by Illumina MiSeq, annotated using RAST and manually curated. The presence of a unique plasmid of 34096-bp and 50 CDS was observed in the four isolates, displaying only 1 nucleotide substitution translated into one amino acid change among them. These plasmids have a class 1 integron containing the aac-(6′)-Ib gene, a mercury resistance operon region and the relE/stbE toxin/antitoxin system. Plasmids showed 79% similarity and 99% identity with pmatvim-7 from Pseudomonas aeruginosa. This is the first full description and characterization of a plasmid from A. ruhlandii which was maintained over time. |
| Databáze: | OpenAIRE |
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