Description of Erythrobacter mangrovi sp. nov., an aerobic bacterium from rhizosphere soil of mangrove plant (Kandelia candel)
| Autor: | Min Wu, Maripat Xamxidin, Nusratgul Anwar, Zhe Zhao, Yanghui Ye, Cen Yan, Yanfang Nie, Ran Zhang, Cong Sun |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences Erythrobacter lutimaris RNA Ribosomal 16S Botany DNA Barcoding Taxonomic Data Mining Molecular Biology Phylogeny Soil Microbiology Rhizosphere biology Phylogenetic tree Strain (chemistry) Computational Biology Kandelia candel Genomics General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Sphingomonadaceae 030104 developmental biology Erythrobacter pelagi Rhizophoraceae Genome Bacterial Bacteria |
| Zdroj: | Antonie van Leeuwenhoek. 113:1425-1435 |
| ISSN: | 1572-9699 0003-6072 |
| Popis: | A novel Gram-stain negative, aerobic, non-motile, rod-shaped bacterium, designated as strain EB310T, was isolated from rhizosphere soil of mangrove plant Kandelia candel in Fugong village, Zhangzhou, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EB310T belonged to the genus Erythrobacter, clustering with Erythrobacter pelagi JCM 17468T, Erythrobacter lutimaris KCTC 42109T and Erythrobacter marisflavi KCTC 62896T, and showed the highest 16S rRNA gene sequence similarity of 97.5% to Erythrobacter pelagi JCM 17468T. The genomic average nucleotide identity and in silico DNA–DNA hybridization values between strain EB310T and the reference strains were 71.0–75.5% and 19.8–20.0%, respectively. Growth ranges of the isolate occurred at 10–45 °C (optimum 28–30 °C), pH 5.5–9.5 (optimum pH 7.5) and 0–9.0% NaCl concentrations (optimum 2.0%, w/v). The strain did not produce bacteriochlorophyll a and flexirubin, but produced carotenoids. The strain contained Q-10 as the predominant ubiquinone and summed feature 3 (C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω6c/C18:1 ω7c) as the major fatty acids. The major polar lipids were sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylcholine. Differential phenotypic characteristics, together with chemotaxonomic, phylogenetic and genomic distinctiveness, indicated that strain EB310T is distinguishable from other members of the genus Erythrobacter. On the basis of the data exhibited, strain EB310T is considered to represent a novel species of the genus Erythrobacter, for which the name Erythrobacter mangrovi sp. nov., is proposed. The type strain is EB310T (= KCTC 72109T = MCCC 1K03690T). The genomic DNA G + C content is 62.9 mol%. |
| Databáze: | OpenAIRE |
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