Low frequency of anti-SLA/LP autoantibody in Japanese adult patients with autoimmune liver diseases: analysis with recombinant antigen assay
| Autor: | Kentaro Kikuchi, Takashi Kato, Wolfgang Schlumberger, Hiroshi Miyakawa, Yumi Kawashima, Eriko Kitazawa, Hirotoshi Fujikawa, Etsuko Hashimoto, Erika Imai, Naomi Kawaguchi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
Immunology Blotting Western Enzyme-Linked Immunosorbent Assay Autoimmune hepatitis medicine.disease_cause Autoantigens Autoimmunity Primary sclerosing cholangitis Autoimmune Diseases Primary biliary cirrhosis Antigen Japan immune system diseases medicine Immunology and Allergy Humans Autoantibodies Hepatitis Autoimmune disease business.industry Liver Diseases fungi Autoantibody Middle Aged medicine.disease Female business |
| Zdroj: | Journal of autoimmunity. 21(1) |
| ISSN: | 0896-8411 |
| Popis: | Anti-soluble liver antigen/liver pancreas (SLA/LP) autoantibody has been proposed to be one of the autoantibodies characterizing autoimmune hepatitis (AIH). Recently, one of the autoantigens to anti-SLA/LP was identified as a UGA suppressor tRNA-associated protein. Although the function of this protein remains unknown, the recombinant protein has been prokaryotically expressed. Using this protein as an antigen, a recombinant immunoassay for anti-SLA/LP autoantibody has been established and the frequency and significance of this autoantibody have been discussed in European countries. So, in the present study, we investigated anti-SLA/LP autoantibodies in Japanese patients with autoimmune liver diseases using the recombinant antigen ELISA and Western blot assay. Seventy-five patients with AIH type 1, 5 with AIH type 2, 46 with primary biliary cirrhosis, 10 with primary sclerosing cholangitis, 47 with chronic hepatitis C, 48 with systemic lupus erythematosus, 3 with cryptogenic hepatitis, and 40 normal controls were the subjects of the present study. Anti-SLA/LP autoantibodies were detected in only 5 of 75 (6.7%) patients with AIH type 1, but in none of the other 159 patients or 40 normal controls. The clinicopathologic features of anti-SLA/LP-positive AIH type 1, including carriers of HLA DR locus variations, were not significantly different from anti-SLA/LP-negative patients except for the mortality rate. Anti-SLA/LP autoantibody was detected at a low frequency in Japanese patients with AIH type 1 and did not significantly influence clinical features. However, since it has high disease-specificity to AIH type 1, further analysis of SLA/LP may contribute to help clarify the pathogenesis of AIH type 1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect