Lectin reactivity of expanded mouse blastocysts after exposure to sera from women with unexplained recurrent spontaneous abortion
| Autor: | Tahereh Talaei-Khozani, Najmeh Aminizadeh, Elham Aliabadi, S Fakhroddin Mesbah A, Jaleh Zolghadr |
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| Rok vydání: | 2005 |
| Předmět: |
Serum
Peanut agglutinin Abortion Habitual medicine.medical_specialty Time Factors Wheat Germ Agglutinins Glycoconjugate Ursa Toxicology Embryo Culture Techniques Mice Peanut Agglutinin Pregnancy Lectins Internal medicine medicine Animals Conceptus Blastocyst reproductive and urinary physiology chemistry.chemical_classification Mice Inbred BALB C biology Lectin Embryo biology.organism_classification Wheat germ agglutinin Abortion Spontaneous medicine.anatomical_structure Endocrinology chemistry embryonic structures biology.protein Female Plant Lectins |
| Zdroj: | Reproductive Toxicology. 20:531-537 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2005.04.015 |
| Popis: | Embryotoxic factors existing in maternal sera may influence their effects via specific binding to, or alteration of cell surface molecules in the conceptus. This study was undertaken to determine the effects of sera from women with unexplained recurrent spontaneous abortion (URSA) on cell surface glycoconjugates of the early conceptus. Four cell stage embryos were cultured in medium supplemented with sera from women with URSA, from normal women, or in medium without serum. Developmental competence was assessed as the stage distribution of embryos advancing to during 96 h in culture. Hatched (expanded) blastocysts were stained with wheat germ agglutinin (WGA), peanut agglutinin (PNA) and dolichos biflorus agglutinin (DBA) to detect surface glucoconjugates. We observed that patient sera could be divided into high- and low-risk groups on the basis of the ability to decrease the number of four-cell embryos reaching the expanded blastocyst stage. Furthermore, the intensity of reactivity to PNA changed after exposure to high-risk sera. Morula formation was reduced and blastocyst formation was delayed. Although the sera from women with URSA had embryotoxic effects, no influence on the glycoconjugate patterns were evident in hatched blastocysts, aside from PNA reactivivity. We suggest altered developmental display of PNA-reactive proteins was a biomarker for poor developmental quality due to emrbyotoxic factors in serum from URSA patients. |
| Databáze: | OpenAIRE |
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