Cloning and Functional Expression of a cDNA Encoding a Human Type 2 Neuropeptide Y Receptor
| Autor: | Patricia M. Rose, Bernadette Kienzle, Krishna Kodukula, Jean S. Lynch, Scott T. Frazier, Prabhavathi B. Fernandes, Susan M. Fisher, Ramakrishna Seethala |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary Neuropeptide Y receptor Y1 Neuropeptide Y receptor Y2 Molecular Sequence Data Restriction Mapping Gene Expression Neuropeptide FF receptor CHO Cells Biology Transfection Binding Competitive Second Messenger Systems Biochemistry Cell Line Cricetinae Chlorocebus aethiops Cyclic AMP Animals Humans Neuropeptide Y 5-HT5A receptor Amino Acid Sequence RNA Messenger Cloning Molecular Receptor Molecular Biology Gene Library Binding Sites Base Sequence Colforsin Cell Biology Blotting Northern Neuropeptide Y receptor Molecular biology Recombinant Proteins Receptors Neuropeptide Y Kinetics Interleukin-21 receptor Expression cloning |
| Zdroj: | Journal of Biological Chemistry. 270:22661-22664 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.270.39.22661 |
| Popis: | Neuropeptide Y (NPY) is a 36-amino acid polypeptide that is widely distributed in the central nervous system and periphery. Pharmacological studies have suggested that there are at least three receptor subtypes, Y1, Y2, and Y3. Cloning of the Y1 subtype has been reported previously. Here we report the isolation by expression cloning of a cDNA encoding a human NPY receptor displaying a pharmacology typical of a Y2 receptor. COS-7 cells transfected with the cDNA express high affinity binding sites for NPY, peptide YY, and NPY13-36, whereas [Leu31,Pro34]NPY binds with lower affinity. The receptor is 381 amino acids in length and has seven putative transmembrane regions typical of G-protein-coupled receptors. Comparison of the amino acid sequence of this Y2 receptor to that of the human Y1 receptor indicates that the two receptors are 31% identical at the amino acid level. Northern blot analyses reveal a single 4-kilobase mRNA species and indicate that the messenger RNA is present in many areas of the central nervous system. NPY induced calcium mobilization and inhibited forskolin-stimulated cAMP accumulation in Chinese hamster ovary cells that stably express the Y2 receptor cDNA, indicating that the recombinant Y2 receptor is functionally coupled to second messenger systems. |
| Databáze: | OpenAIRE |
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