Somatic embryogenesis in cultured immature kernels of Pistachio, Pistacia vera L

Autor:	Michael M. Yeoman, Ahmet Onay, C. E. Jeffree
Rok vydání:	1995
Předmět:	Pistacia Somatic embryogenesis Plant Science General Medicine Biology biology.organism_classification chemistry.chemical_compound Tissue culture chemistry Germination Botany Cytokinin Anacardiaceae Agronomy and Crop Science Fruit tree Explant culture
Zdroj:	Plant Cell Reports. 15:192-195
ISSN:	1432-203X 0721-7714
DOI:	10.1007/bf00193718
Popis:	Embryogenic tissue was produced from kernels of immature fruits of Pistachio (Pistacia vera L.) cultured in liquid Murashige and Skoog media, supplemented with 200 mgl−1 casein hydrolysate, 114 μM 1-ascorbic acid, and benzylaminopurine. Compact embryogenic masses differentiated directly from the fruit explants after culture for 2 weeks in liquid medium with 8.9 μM benzylaminopurine. After transfer of the embryogenic masses into the same medium, but with 4.4 μM benzylaminopurine, somatic embryos appeared. Several stages of embryogenesis were present in the cultures. Adventive embryos were readily separated from the friable embryogenic masses by shaking. Separated somatic embryos, germinated on solidified Murashige & Skoog medium without growth regulators, developed into plantlets.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e135a17508665395319c2ee1ceeb3643 https://doi.org/10.1007/bf00193718 Zobrazit plný text záznamu Full text from SpringerLink