A method for expansion of T cells from cynomolgus monkey (Macaca fascicularis)
| Autor: | Qian Song, Miao Yi, Jian Dong, Change Gao, Jian Li, Ming Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment T cell CD3 T-Lymphocytes Cell Culture Techniques Biology 03 medical and health sciences Antigen Cancer immunotherapy medicine Animals Humans Cell Proliferation Cell growth Cell Biology General Medicine Immunotherapy Molecular biology Macaca fascicularis 030104 developmental biology medicine.anatomical_structure Phenotype Cell culture biology.protein Stem cell K562 Cells Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 54(8) |
| ISSN: | 1543-706X |
| Popis: | T cells have been successfully applied to cancer immunotherapy. However, a challenge is an expansion of T cells from cynomolgus monkey, which is a valuable non-human primate model for T cell therapy transferring to the clinic. Here, we compared several strategies to expand cynomolgus monkey T cell and developed an appropriate method. Our study demonstrated that 100 ng/ml CD3 mAb + 1% PHA+ 1000 U/ml IL2 therapy significantly expanded peripheral blood CD3+ T cells without compromising T cell phenotype in vitro. The results of this study could be used for T cell remodeling, which has significant therapeutic potential in Chimeric Antigen Receptor-T (CAR-T) cell immunotherapy. |
| Databáze: | OpenAIRE |
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